Copper-cysteamine nanoparticle-mediated microwave dynamic therapy improves cancer treatment with induction of ferroptosis

作     者：Hui Zhou Zhongtao Liu Zijian Zhang Nil Kanatha Pandey Eric Amador William Nguyen Lalit Chudal Li Xiong Wei Chen Yu Wen 

作者机构：Department of General SurgerySecond Xiangya HospitalCentral South UniversityChangsha410011China Department of PhysicsThe University of Texas at ArlingtonArlingtonTX76019-0059USA 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第24卷第6期

页      面：322-330页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 0805[工学-材料科学与工程（可授工学、理学学位）] 100214[医学-肿瘤学] 10[医学] 

基　　金：the support by the Natural Science Foundation of China(81773293,81873640,81970569,82000756) Natural Science Foundation of Hunan Province,No.2022JJ40700 the Key Project of Science and Technology Program of Hunan Provincial Science and Technology Department(2015GK3117,2017WK2063) the supports from Guangxi Jialouyuan Medical Inc.,Solgro the distinguished award from UT Arlington as well as ROSFORCURE Inc 

主　　题：Cu-Cy Microwave PDT Cell death Colorectal cancer Ferroptosis 

摘      要：Photodynamic Therapy(PDT)holds a great promise for cancer patients,however,due to the hypoxic characteristics of most solid tumors and the limited penetration depth of light in tissues,the extensive clinical application of PDT is ***,we report microwave induced copper-cysteamine(Cu-Cy)nanoparticles-based PDT as a promising cancer treatment to overcome cancer resistance in combination with *** treatment efficiency of Cu-Cy-mediated microwave dynamic therapy(MWDT)tested on HCT15 colorectal cancer(CRC)cells via cell titer-blue cell viability assay and live/dead assay reveal that Cu-Cy upon MW irradiation can effectively destroy HCT15 CRC cells with average IC-50 values of 20μg/*** cytotoxicity of Cu-Cy to tumor cells after MW stimulation can be alleviated by ferroptosis ***,Cu-Cy mediated MWDT could deplete glutathione peroxide 4(GPX4)and enhance lipid peroxides(LPO)and malondialdehyde(MDA).Our findings demonstrate that MW-activated Cu-Cy killed CRC cells by inducing *** superior in vivo antitumor efficacy of the Cu-Cy was corroborated by a HCT15 tumor-bearing mice *** experiments showed that the GPX4 expression level in Cu-Cy+MW group was significantly lower than that in other ***,these findings demonstrate that Cu-Cy nanoparticles have a safe and promising clinical application prospect in MWDT for deep-seated tumors and effectively inhibit tumor cell proliferation by inducing ferroptosis,which provides a potential solution for cancer resistance.