Oxygen metabolism-balanced engineered hydrogel microspheres promote the regeneration of the nucleus pulposus by inhibiting acid-sensitive complexes

作     者：Ziang Li Feng Cai Jincheng Tang Yichang Xu Kaijin Guo Zonghan Xu Yu Feng Kun Xi Yong Gu Liang Chen 

作者机构：Department of Orthopedicsthe First Affiliated Hospital of Soochow UniversityOrthopedic InstituteSoochow University188 Shizi RoadSuzhouJiangsu215006PR China Department of Orthopedicsthe Affiliated Hospital of Xuzhou Medical University99 Huaihai West RoadXuzhouJiangsu221000PR China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第24卷第6期

页      面：346-360页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：supported by the National Natural Science Foundation of China(81972078,82120108017,82072438,82102589,81702190) Social Development Project of Jiangsu Province(BE2021646),Standardized Diagnosis and Treatment Project of Key Diseases in Jiangsu Province(BE2015641) the Natural Science Foundation of Jiangsu Province(BK20211504 and BK20170370) Suzhou Gusu Health Talent Program(GSWS2020001 and GSWS2021007) Jiangsu Innovative and Entrepreneurial Talent Program(JSSCBS20211570) Medical Health Science and Technology Innovation Program of Suzhou(SKY2022119) 

主　　题：Antioxidant stress Microsphere BPQDs IVDD Reductive agent 

摘      要：Intervertebral disc degeneration(IVDD)is commonly caused by imbalanced oxygen metabolism-triggered *** the shortcomings of antioxidants in IVDD treatment,including instability and the lack of targeting,remains *** and surface modification technologies were combined to graft chitosan nanoparticles encapsulated with strong reductive black phosphorus quantum dots(BPQDs)onto GelMA microspheres via amide bonds to construct oxygen metabolism-balanced engineered hydrogel microspheres(GM@CS-BP),which attenuate extracellular acidosis in nucleus pulposus(NP),block the inflammatory cascade,reduce matrix metalloproteinase expression(MMP),and remodel the extracellular matrix(ECM)in intervertebral discs(IVDs).The GM@CS-BP microspheres reduce H_(2)O_(2) intensity by 229%.Chemical grafting and electrostatic attraction increase the encapsulation rate of BPQDs by 167%and maintain stable release for 21 days,demonstrating the antioxidant properties and sustained modulation of the *** the GM@CS-BP treatment,western blotting revealed decreased acid-sensitive ion channel-3 and inflammatory *** staining in an 8-week IVDD model confirmed the regeneration of ***@CS-BP microspheres therefore maintain a balance between ECM synthesis and degradation by regulating the positive feedback between imbalanced oxygen metabolism in IVDs and *** study provides an in-depth interpretation of the mechanisms underlying the antioxidation of BPQDs and a new approach for IVDD treatment.