与标准古DNA实验方案相关的污染物评估

作     者：苗波 刘雅琳 杨若薇 冯晓恬 刘峰 曹鹏 戴情燕 平婉菁 刘逸宸 付巧妹 Bo Miao;Yalin Liu;Ruowei Yang;Xiaotian Feng;Feng Liu;Peng Cao;Qingyan Dai;Wanjing Ping;Yichen Liu;Qiaomei Fu

作者机构：College of Life SciencesNorthwest UniversityXi’an 710069China Key Laboratory of Vertebrate Evolution and Human OriginsInstitute of Vertebrate Paleontology and PaleoanthropologyCenter for Excellence in Life and PaleoenvironmentChinese Academy of SciencesBeijing 100044China Shanghai Qi Zhi InstituteShanghai 200232China University of Chinese Academy of SciencesBeijing 100049China 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2023年第68卷第1期

页      面：5-9页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported by the Chinese Academy of Sciences(YSBR-019 and XDB26000000) the National Natural Science Foundation of China(41925009 and T2192950) the Tencent Foundation(through the XPLORER PRIZE) the Howard Hughes Medical Institute(55008731) the Feng Foundation of Biomedical Research the Shanghai Qi Zhi Institute supported by Special research assistants funded project of the Chinese Academy of Sciences 

主　　题：automation Ancient library 

摘      要：Ancient DNA(aDNA)techniques have rapidly evolved in the past decade,including the introduction of next-generation sequencing(NGS)techniques,the modification and optimization of various protocols to extract highly degraded DNA,and the incorporation of these molecules into sequencing libraries[1].Two major milestones are the application of single-stranded DNA library construction protocol and the automation of lab work using liquid handling robots[1].