Variation of photosynthesis,secondary metabolites and antioxidant activities in third generation of spaceflight-induced Salvia miltiorrhiza

作     者：Liang Peng Mei Ru Zongsuo Liang Liang Pen;Mei Ru;Zongsuo Liang

作者机构：College of PharmacyShaanxi University of Chinese MedicineXi’an 710000China College of Life Sciences and MedicineZhejiang Sci-Tech UniversityHangzhou 310000China College of Life SciencesNorthwest A&F UniversityYangling 712100China College of PharmacyGuangxi University of Chinese MedicineNanning 530200China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2022年第14卷第4期

页      面：592-601页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：supported by grants from the Twelfth Five-Year Plan for Science&Technology Support of China(No.2015BAC01B03) the National Natural Science Foundation of China(No.81373908)。 

主　　题：antioxidative ability Salvia miltiorrhiza Bge. secondary metabolism spaceflight third generation primary metabolism 

摘      要：Objective: Spaceflight has long been perceived as an effective way to improve the quantity and quality of plants with wide applications. In order to obtain stable and inheritable descendants of spaceflightinduced Salvia miltiorrhiza lines, we investigated and analyzed four lines m16, m50, m51, m57(three individuals of each line) and the ground control(three individuals) of the third generation of spaceflight-induced S. miltiorrhiza from primary/secondary metabolism and antioxidative abilities.Methods: A portable photosynthesis system(Li-6400) with red/blue LED light source was used to perform the photosynthetic characteristics to evaluate their primary productivity. The secondary metabolites(phenolic acids, tanshinones, total phenolics and flavonoids) and antioxidant activity of roots were analyzed to assess their quality.Results: Compared with control, line m16 presented weak photosynthetic ability, but high apparent quantum yield(AQY), higher contents of secondary metabolites, and stronger antioxidative abilities.Line m57 had a strong gas exchange ability, relatively higher secondary metabolites contents, and ascending antioxidative abilities. Lines m50 and m51 were in the middle level of lines m16 and m57.The principal component analysis for all the original data revealed three components including a rootrelated index, a leaf-related index, and a CO_(2) response parameter could be used to distinguish spaceflight-induced S. miltiorrhiza lines.Conclusion: Line m57 could be an appropriate material for the investigation of targeted breeding towards high production, and line m16 could be used to identify essential genes and unravel sophisticated pathways underlying the secondary metabolisms.