Generation and characterization of novel stromal specific antibodies

作     者：Sapna HALDER,Debbie L. HARDIE,Dagmar SCHEEL-TOELLNER,Mike SALMON,Christopher D. BUCKLEY 

作者机构：Prostate Cancer Research Centre Institute of Urology (3rd floor) University College London 67 Riding House Street London W1W 7EJ UK Rheumatology Research Group MRC Centre for Immune Regulation Institute of Biomedical Research (3rd or 4th floor) University of Birmingham Birmingham B15 2TT U K Rheumatology Research Group MRC Centre for Immune Regulation Institute of Biomedical Research (3rd or 4th floor) University of Birmingham Birmingham B15 2TT U K Rheumatology Research Group MRC Centre for Immune Regulation Institute of Biomedical Research (3rd or 4th floor) University of Birmingham Birmingham B15 2TT U K Rheumatology Research Group MRC Centre for Immune Regulation Institute of Biomedical Research (3rd or 4th floor) University of Birmingham Birmingham B15 2TT U K 

出 版 物：《Cell Research》 (细胞研究(英文版))

年 卷 期：2005年第9期

页      面：64-69页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：stromal mesenchymal non-haematopoietic rheumatoid fibroblasts CD45. 

摘      要：Rheumatoid synovial fibroblasts were used as an immunogen to produce monoclonal antibodies selected for their reactivity with stromal cell antigens. Mice were immunised with low passage whole cell preparations and the subsequent hybridomas were screened by immunohistochemistry on rheumatoid synovium and tonsil sections. The aim was to identify those antibodies that recognised antigens that were restricted to stromal cells and were not expressed on CD45 positive leucocytes. A significant number of antibodies detected antigen that identified endothelial cells. These antibodies were further characterised to determine whether the vessels identified by these antibodies were vascular or lymphatic. From five fusions clones were identified with predominant reactivity with: 1) fibroblasts and endothelial cells; or 2) broad stromal elements (fibroblast, endothelium, epithelium, follicular dendritic cells). A fibroblast-specific antibody that did not also identify vessels was not generated. Examples of each reactivity pattern are discussed.