Molecular Cloning and Characterization of Human Homeobox Gene Nkx3.1 Promoter

作     者：An-Li JIANG, Jian-Ye ZHANG, Charles YOUNG, Xiao-Yan HU, Yong-Mei WANG, Zhi-Fang LIU, and Mei-Lan HAO ( Department of Biochemistry, School of medicine, Shandong University, Ji'nan 250012, China ' Department of Urology Research, Mayo Clinic ) Present address: Department of biochemistry, Health School of Ji'nan 

作者机构：Department of Biochemistry School of medicine Shandong University Ji'nan 250012 China Department of Urology Research Mayo Clinic 

出 版 物：《Acta Biochimica et Biophysica Sinica》 (生物化学与生物物理学报(英文版))

年 卷 期：2004年第36卷第1期

页      面：64-67页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：This work was supported by a grant from the National Natural Science Foundation of China (No. 30171026) 

主　　题：homeobox gene Nkx3.1 promoter deletion mutagenesis LNCaP cell line 

摘      要：Nkx3.l is a prostate-specific homeobox gene related strongly to prostate development and prostate cancer. To study its regulation of transcription, 1.06 kb 5 flanking region of Nkx3.1 gene and its 5 deletion mutants (861, 617, 417 and 238 bp) were obtained by PCR and cloned into pGL3-basic, a promoter-less luciferase reporter vector, to examine their promoter activities driving the reporter gene transcription. pRL-TK, a Renilla luciferase reporter vector was used as internal control, and pGL3-control and pGL3-basic were used as positive and negative control respectively. The promoter activities were determined by dual-luciferase reporter assay 48 h after pGL3 constructs were cotransfected with pRL-TK into prostate cancer cell LNCaP. The results showed that dual-luciferase reporter assay (M1/M2) of pGL3-1.06 kb cotransfection with pRL-TK was 2.7, which was about 1.5-fold higher than that of pGL3-control cotrans-fection with pRL-TK and 50-fold higher than that of pGL3-basic cotransfection with pRL-TK. T