Related gene expressions in anti-keratinocyte aging induced by Ganoderma lucidum polysaccharides

作     者：Xie Shaoqiong Liao Wanqing Yao Zhirong Wang Zhidong 

作者机构：Department of Dermatology Shangai Skin Disease and STD Shanghai 200050 China Department of Dermatology Changzheng Hospital Second Military Medical University Shanghai 200003 China 

出 版 物：《Journal of Medical Colleges of PLA(China)》 (中国人民解放军军医大学学报（英文版）)

年 卷 期：2008年第23卷第3期

页      面：167-175页

学科分类：0710[理学-生物学] 07[理学] 071008[理学-发育生物学] 

基　　金：the CMA–L’OREALCHINA SKIN GRANT (2005) 

主　　题：Ganoderma lucidum polysaccharides Skin aging Gene chip Keratinocyte 

摘      要：Objective:To examine the level of expression of anti skin aging gene of Ganoderma lucidum polysacchayides and clarify its mechanism with anti aging of this ancient Chinese ***:HacaT cell of keratinocytes lines were cultured and treated with the *** total RNA was extracted with Trizol reagent and cDNA was synthesized by reverse *** obtained cDNAs were then fluorescently labeled with cy3 and cy5 respectively and hybridized with gene expressing pedigree cDNA *** images were scanned and analyzed with special *** scan data were analyzed with software and checked by real time ***:Among total 18 346 human genes,the expression of 103 ones was up-regulated and 378 ones *** was demonstrated evidently that Ganoderma lucidum polysaccharides affected the expression of genes of anti skin *** ways are ***:it is concluded by analysis of function of these up-regulation and down-regulation genes that Ganoderma lucidum polysaccharides may play an important role in boosting cell growth and against skin *** shows that the results of gene array reliable by real time PCR.