Immunohistochemical study of the digestive tract of Oligosarcus hepsetus
Immunohistochemical study of the digestive tract of Oligosarcus hepsetus作者机构:Departamento de Biologia AnimalInstituto de Biologia Universidade Federal Rural do Rio de Janeiro
出 版 物:《World Journal of Gastroenterology》 (世界胃肠病学杂志(英文版))
年 卷 期:2013年第19卷第12期
页 面:1919-1929页
核心收录:
学科分类:1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学]
基 金:Supported by Federal Rural University of Rio de Janeiro for the Research Grant under the Internal Scientific Initiation Program to Vieira-Lopes DA
主 题:Fish Esophagus Stomach Intestine Endocrine cells
摘 要:AIM: To describe the histology of the digestive tract and to investigate the occurrence of endocrine cells in Oligosarcus hepsetus (O. hepsetus ). METHODS: The digestive tract (DT) of O. hepsetus was divided into esophagus, two stomach regions (glandular and non-glandular) and two intestinal regions (anterior and posterior). These specimens were processed by routine histological techniques and stained with hematoxylin-eosin, Gomori s trichrome, periodic acid Schiff (PAS) and Alcian blue (AB). An immunohistochemical method using avidin-biotin-peroxidase was ***: The esophagus is lined with a non-keratinized stratified squamous epithelium that is reactive to PAS and AB. The stomach has a mucosa lined with a simple columnar epithelium with mucus-secreting cells that are reactive only to PAS. The intestine has a simple columnar epithelium with a brush border and goblet cells that are reactive to PAS and AB. Somatostatin, serotonin and cholecystokinin immunoreactive cells were identified throughout the ***: This study revealed adaptations for the species diet and showed that the distribution and relative frequency of immunoreactive cells are similar to those of other fish.