Base editing-mediated targeted evolution of ACCase for herbicide-resistant rice mutants

作     者：Hongzhi Wang Yuxin He Yingying Wang Zuren Li Jiannan Hao Yijiao Song Mugui Wang Jian-Kang Zhu Hongzhi Wang;Yuxin He;Yingying Wang;Zuren Li;Jiannan Hao;Yijiao Song;Mugui Wang;Jian-Kang Zhu

作者机构：Shanghai Center for Plant Stress Biology and Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 201602China University of Chinese Academy of SciencesBeijing 100049China Hunan Provincial Key Laboratory for Biology and Control of WeedsHunan Academy of Agricultural SciencesChangsha 410125China College of Life SciencesNanjing Agricultural UniversityNanjing 210095China Institute of Advanced Biotechnology and School of Life SciencesSouthern University of Science and TechnologyShenzhen 518055China Center for Advanced Bioindustry TechnologiesChinese Academy of Agricultural SciencesBeijing 100081China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2022年第64卷第11期

页      面：2029-2032页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 

基　　金：funded by the National Natural Science Foundation of China(31901046) Youth Innovation Promotion Association,Chinese Academy of Science(2020272)。 

主　　题：evolution enable editing 

摘      要：Base editing technologies enable precise base alterations in a target gene without inducing double-stranded breaks,and thus are powerful for targeted gene evolution in vivo(Zhan et al.,2021).The newly evolved cytosine deaminases such as evoAPOBEC1,evoFERNY and evoCDA1,adenine deaminase TadA8e,and a near-protospacer adjacent motif(PAM)less SpRYCas9 variant,have greatly increased base editing efficiency and expanded the editing window in plants(Zhang et al.,2021;Xu et al.,2021c;Tan et al.,2022).Dual cytosine and adenine editors enable concurrent C-to-T and A-to-G conversions and thus enrich the editing outcome.Although plant dual editors have been developed(Li et al.,2020;Xu et al.,2021a),they are rarely applied in evolving plant genes due to low efficiencies.