Phosphorylation of CENP-R by Aurora B regulates kinetochore-microtubule attachment for accurate chromosome segregation

作     者：Divine Mensah Sedzro Xiao Yuan McKay Mullen Umer Ejaz Tongtong Yang Xu Liu Xiaoyu Song Yun-Chi Tang Weijun Pan Peng Zou Xinjiao Gao Dongmei Wang Zhikai Wang Zhen Dou Xing Liu Xuebiao Yao Divine Mensah Sedzro;Xiao Yuan;McKay Mullen;Umer Ejaz;Tongtong Yang;Xu Liu;Xiaoyu Song;Yun-Chi Tang;Weijun Pan;Peng Zou;Xinjiao Gao;Dongmei Wang;Zhikai Wang;Zhen Dou;Xing Liu;Xuebiao Yao

作者机构：MOE Key Laboratory for Cellular DynamicsUniversity of Science and Technology of China School of Life SciencesHefei 230027China Anhui Key Laboratory for Cellular Dynamics and Chemical BiologyNational Center for Cross-Disciplinary Sciences&CAS Center for Excellence in Molecular Cell ScienceHefei 230026China Morehouse School of MedicineAtlantaGA 30310USA Shanghai Institute of Nutrition and HealthShanghai Institutes for Biological SciencesChinese Academy of Sciences(CAS)Shanghai 200031China College of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China 

出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)

年 卷 期：2022年第14卷第7期

页      面：62-75页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Ministry of Science and Technology of China(MOST)grants(2017YFA0503600) National Natural Science Foundation of China(NSFC)grants(91854203,31621002,91853115,21922706,92153302,32090040,22177106,31871359,92053104,32100612,22137007,and 31970655) Ministry of Education(IRT_17R102,20113402130010,and YD2070006001) Strategic Priority Research Program of the Chinese Academy of Sciences(XDB19040000) Anhui Provincial Natural Science Foundation Grant(2108085J15 and 1908085MC64) Fundamental Research Funds for the Central Universities(WK2070000066 and WK2070000194) 

主　　题：constitutive centromere-associated network(CCAN) kinetochore microtubule Aurora B CENP-R phosphorylation 

摘      要：Error-free mitosis depends on accurate chromosome attachment to spindle microtubules via a fine structure called the centromere that is epigenetically specified by the enrichment of CENP-A *** maintenance during mitosis requires CENP-A-mediated deposition of constitutive centromere-associated network that establishes the inner kinetochore and connects centromeric chromatin to spindle microtubules during *** previously proposed to be an adaptor of retinoic acid receptor,here,we show that CENP-R synergizes with CENP-OPQU to regulate kinetochore-microtubule attachment stability and ensure accurate chromosome segregation in *** found that a phospho-mimicking mutation of CENP-R weakened its localization to the kinetochore,suggesting that phosphorylation may regulate its *** of CENP-R phosphorylation is shown to prevent proper kinetochore-microtubule attachment at ***,CENP-R phosphorylation disrupts its binding with ***,we speculate that Aurora B-mediated CENP-R phosphorylation promotes the correction of improper kinetochore-microtubule attachment in *** CENP-R is absent from yeast,we reasoned that metazoan evolved an elaborate chromosome stability control machinery to ensure faithful chromosome segregation in mitosis.