Production of bacterial blight resistant lines from somatic hybridization between Oryza sativa L. and Oryza meyeriana L.

作     者：严成其 钱凯先 薛刚平 吴忠长 陈跃磊 颜秋生 张雪琴 吴平 

作者机构：CollegeofLifeSciencesZhejiangUniversityHangzhou310029China CSIROPlantIndustry306CarmodyRd.StLuciaQld4067Australia ChinaNationalRiceResearchInstituteHangzhou310006China 

出 版 物：《Journal of Zhejiang University Science》 (浙江大学学报（自然科学英文版）)

年 卷 期：2004年第5卷第10期

页      面：1199-1205页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 0901[农学-作物学] 090402[农学-农业昆虫与害虫防治] 

基　　金：Ningbo Agriculture Key Scientific Research Foundation  China 

主　　题：Oryza sativa L. Oryza meyeriana L. Somatic hybridization Rice bacterial blight resistance 

摘      要：Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both *** and ***. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.