Integration of network pharmacology with experimental verification reveals the hypoglycemic mechanism of coptisine in Jinqi Jiangtang tablets:inhibition of the FoxO1 signaling pathway and hepatic gluconeogenesis
作者机构：School of Chinese Materia Medica/School of Traditional Chinese MedicineBeijing University of Chinese MedicineBeijing 100029China State Key Laboratory of Bioactive Substance and Function of Natural MedicinesInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China New Drug Safety Evaluation CenterInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China Beijing Union-Genius Pharmaceutical Technology Development Co.Ltd.Beijing 100176China
出 版 物：《Traditional Medicine Research》 (TMR传统医学研究)
年 卷 期：2023年第8卷第3期
页 面：51-61页
学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学]
基 金：the Fundamental Research Funds for the Central Universities(grant number:2021-JYB-XJSJJ-003) the Open Project of State Key Laboratory of Bioactive Substance and Function of Natural Medicines(grant number:GTZK202108) Chinese Society of Toxicology(grant number:CST2021CT101) Discipline Construction Project of Peking Union Medical College(grant number:201920200801)
主 题：Jinqi Jiangtang tablets FoxO1 gluconeogenesis type 2 diabetes mellitus network pharmacology
摘 要：Background:Jinqi Jiangtang tablets(JQJT)have been approved for the treatment of type 2 diabetes mellitus(T2DM)in China for many *** the effective substances and mechanisms of JQJT is important for its clinical application and further drug research and *** study aimed to explore the chemical basis and mechanisms of JQJT in the treatment of ***:With network pharmacology,we screened substances in JQJT and their possible targets,then constructed the action network and enriched the biological functions and pathways associated with the active components,and identified the potential targets and mechanisms of JQJT in the treatment of *** on the network pharmacology data,we explored the hypoglycemic mechanisms of coptisine in JQJT through western blot and quantitative real-time polymerase chain ***:Forty-three compounds with good pharmacokinetic properties were identified in JQJT,together with 146 potential biological *** these potential targets,74 were associated with treatment of T2DM.A compound-target network of the 43 compounds against T2DM was *** process and signal pathway enrichment analysis of the network highlighted the FoxO signaling *** blot and quantitative real-time polymerase chain reaction results showed that coptisine,but not epiberberine,significantly inhibited expression of key genes involved in hepatocyte gluconeogenesis by regulating the FoxO1 signaling ***:Network pharmacology analysis and cell experiments showed that coptisine regulated glucose homeostasis by inhibiting the FoxO1 signaling pathway and hepatic gluconeogenesis,which may be one of the mechanisms of JQJT in the treatment of T2DM.
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