The anti-cancerous mechanism of licochalcone A on human hepatoma cell HepG2 based on the miRNA omics

作     者：Jun Wang Xiuxiu Zhang Zhijing Ni Elnur Elam Kiran Thakur Kexin Li Chuyan Wang Jianguo Zhang Zhaojun Wei Jun Wang;Xiuxiu Zhang;Zhijing Ni;Elnur Elam;Kiran Thakur;Kexin Li;Chuyan Wang;Jianguo Zhang;Zhaojun Wei

作者机构：School of Biological Food and EnvironmentHefei UniversityHefei 230601China School of Food and Biological EngineeringHefei University of TechnologyHefei 230009China School of Biological Science and EngineeringNingxia Key Laboratory for the Development and Application of Microbial Resources in Extreme EnvironmentsNorth Minzu UniversityYinchuan 750021China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2023年第12卷第4期

页      面：1136-1148页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the Hefei University Scientific Research and Development Fund(20ZR09ZDB) the talent fund of Hefei University(20RC48) the University Natural Sciences Research Project of Anhui Province(KJ2021A1009) the Major Projects of Science and Technology in Anhui Province(201903a06020021,202004a06020042,202004a06020052,201904a06020008) the National Natural Science Foundation of China(31850410476). 

主　　题：Licochalcone A HepG2 cells Dysregulated miRNAs Transcription factors Targets Regulatory networks 

摘      要：To explore the function of licochalcone A as an anticancer phytochemical on HepG2 cells and investigate its potential mechanisms,we analyzed the microRNAs(miRNAs)expression profile of HepG2 cells in response to licochalcone A(70μmol/L)in vitro.102 dysregulated miRNAs were detected,and SP1 was expected as the transcription factor that regulates the functions of most screened miRNAs.A sum of 431 targets,the overlap of predicted mRNAs from TargetScan,miRDB,and miRtarbase were detected as the targets for these dysregulated miRNAs.FoxO signaling pathway was the hub pathway for the targets.A protein-protein interaction network was structured on the STRING platform to discover the hub genes.Among them,PIK3R1,CDC42,ESR1,SMAD4,SUMO1,KRAS,AGO1,etc.were screened out.Afterwards,the miRNA-target networks were established to screen key dysregulated miRNAs.Two key miRNAs(hsa-miR-133b and hsa-miR-145-5p)were filtered.Finally,the miRNA-target-transcription factor networks were constructed for these key miRNAs.The networks for these key miRNAs included three and two transcription factors,respectively.These identified miRNAs,transcription factors,targets,and regulatory networks may offer hints to understand the molecular mechanism of licochalcone A as a natural anticarcinogen.