Jeju seaweeds suppress lipopolysaccharide-stimulated proinflammatory response in RAW 264.7 murine macrophages

作     者：Eun-Jin Yang Ji-Young Moon Sang Suk Kim Kyong-Wol Yang Wook Jae Lee Nam Ho Lee Chang-Gu Hyun 

作者机构：Cosmetic Science CenterDepartment of ChemistryJeju National University Jeju TechnoparkAra-1-dongJeju-siJeju 690-121.Korea Citrus Research StationNational Institute of Horticultural & Herbal ScienceRDAJeju 699-946.Korea Jeju Love Co.Ltd.542-5 Haengwon-ri.Gujwa-eupJeju 695-975.Korea LINC AgencyJeju National University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2014年第4卷第7期

页      面：529-537页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the fostering program of regionally specialized industries through the Korea Institute for Advancement of Technology(KIAT) Jeju Institute for Regional Program Evaluation funded by the Ministry of Trade.Industry and Energy(2013-R0002288) 

主　　题：Nitrie oxide Inlerleukin-6 Prostaglandin E_2 Tumor necrosis factor-α Seaweeds Proinflammatory mediators 

摘      要：Objective:To investigate the anti-inflammatory effects of Jeju seaweeds on macrophage RAW264.7 cells under lipopolysaccharide(LPS)***:Ethyl acetate fractions were prepared from five different types of Jeju seaweeds,Dictyopteris divaricata(***),Dictyopteris prolifera(***),Prioutis cornea(***,Grateloupia laceolata(G,lanceolate,and Cralcloupia filicina(***)They were screened for inhibitory effects on proinflammatory mediators and cytokines such as nitric oxide(NO),prostaglandin E,,tumor necrosis factor-a(TNF-a),and interleukin-6(11.-6).Results:Our results revealed that ***,***,***,***,and *** potently inhibited ***-stimulaled NO production(IC_(50),values were 18.0,38.36,38.43,32.81 and 37.14μg/mL,respectively).Consistent with these findings,***,***,***,and *** also reduced the IPS-induced and prostaglandin E,production in a concentration-dependent ***,they suppressed the expression of inducible NO synthase and cyclooxygenase-2 at the protein level in a dose-dependent manner in the RAW264.7 cells,as detennined by western *** addition,the levels of TNF-a and IL-6,released into the medium,were also reduced by ***,***,***,G,lanceolata,and *** in a dose-dependent manner(IC_(50)values for TNF-a were 16.11,28.21,84.27,45.52 and74.75μg/mL,respectively;IC_(50),values for IL-6 were 37.35,80.08,103.28,62.53 and 84.28μg/mL,respectively).The total phlorotannin content was measured by the Folin-Ciocalteu method and expressed as phloroglucinol *** content was 92.0μg/mg for ***,151.8μg/mg for ***,57.2μg/mg for ***,53.0 pg/mg for ***,and 40.2μg/mg for ***:Thus,these findings suggest that Jeju seaweed extracts have potential therapeutic applications for inflammatory responses.