Facile suspension culture protocol of the liver biliary organoids

作     者：Jin Chen Jiting Zhang Li Yang Bing Zhao Jin Chen;Jiting Zhang;Li Yang;Bing Zhao

作者机构：State Key Laboratory of Genetic EngineeringSchool of Life SciencesHuman Phenome InstituteZhongshan HospitalFudan UniversityShanghai 200438China 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2023年第6卷第1期

页      面：74-81页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by grants from the National Key Research and Development Program of China(2018YFA0109400) the National Natural Science Foundation of China(32022022) the Shanghai Pilot Program for Basic Research—Fudan University 21TQ1400100(21TQ003) 

主　　题：biliary liver Liver 

摘      要：Introduction Why do we focus on human liver biliary organoids?The three-dimensional(3D)organoid approach is emerging as a powerful tool for basic study and biomedical applications due to its excellent recapitulation of the structure and functions of the primary tissues[1-3].Liver biliary organoids are derived from the biliary tree,which includes the intrahepatic bile duct(IHBD)and extrahepatic bile duct(EHBD).