Positive and negative effects of graphite flake and monolayer graphene oxide templates on protein crystallization
作者机构：Department of Chemical EngineeringLoughborough UniversityLoughboroughLE113TUUK Institute of Materials ResearchTsinghua Shenzhen International Graduate SchoolTsinghua UniversityShenzhen518055China Human Sciences Research CentreUniversity of DerbyDE221GBUK
出 版 物：《Particuology》 (颗粒学报(英文版))
年 卷 期：2023年第79卷第8期
页 面：27-34页
核心收录：
学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学]
主 题：Graphite Graphene oxide Protein crystallization Adsorption Heterogeneous nucleation Template technology
摘 要：Heterogeneous template-induced nucleation is a promising way to regulate protein crystallization events and could be employed for purification processes and crystallographic *** crystallization process with graphite and graphene oxide,as heterogeneous templates,were *** than 640 hanging drops with different concentrations of Lysozyme(30,50,70,100 mg/mL)and NaCl(0.7,0.9,1.1,1.3,1.5 M)were crystallised at 4 ℃ with or without graphite/graphene oxide *** induction times and crystallization process were observed under the *** lysozyme in the solutions with graphite flakes nucleated faster under all the conditions than the lysozyme with equal experimental conditions without *** crystals preferred to grow around the edge of graphite flakes than on the flat *** the droplets with monolayer graphene oxide,more crystals appeared around gra-phene oxide particles,and the faster or slower nucleation processes with templates were dependent on the lysozyme and NaCl *** oxide templates strongly inhibited nucleation at high lysozyme concentrations but promoted nucleation at low lysozyme *** heterogeneous templates changed the crystal morphology and the crystallization *** crystals were observed in the solution with graphite templatesthan with graphene oxide templates and without any template.
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