Embelin alleviates weaned piglets intestinal inflammation and barrier dysfunction via PCAF/NF-κB signaling pathway in intestinal epithelial cells
作者机构:Department of Animal Nutrition and Feed ScienceCollege of Animal Science and TechnologyHuazhong Agricultural UniversityWuhan 430070China
出 版 物:《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志(英文版))
年 卷 期:2023年第14卷第4期
页 面:1629-1638页
核心收录:
学科分类:090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学]
基 金:supported by the National Natural Science Foundation of China(Grant no.32072742) National Key Research and Development Program(Grant no.2021YFD1300300) the Fellowship of China Postdoctoral Science Foundation(grant no.2022M711274)
主 题:Acetylation Inflammation Intestinal barrier PCAF Weaned pig
摘 要:Background:Intestinal barrier plays key roles in maintaining intestinal *** damage can severely destroy the intestinal integrity of *** study was conducted to investigate the protective effects of embelin and its molecular mechanisms on intestinal inflammation in a porcine *** hundred sixty 21-day-old castrated weaned pigs(Duroc×Landrace×Yorkshire,average initial body weight was 7.05 d to four groups and fed with a basal diet o±0.28 kg,equal numbers of castrated males and females)were allotter a basal diet containing 200,400,or 600 mg embelin/kg for 28 *** growth performance,intestinal inflammatory cytokines,morphology of jejunum and ileum,tight junctions in the intestinal mucosa of piglets were ***-1 cells with overexpression of P300/CBP associating factor(PCAF)were treated with embelin,the activity of PCAF and acetylation of nuclear factor-κB(NF-κB)were analyzed to determine the effect of embelin on PCAF/NF-κB pathway in ***:The results showed that embelin decreased(P0.05)serum D-lactate and diamine oxidase(DAO)levels,and enhanced the expression of ZO-1,occludin and claudin-1 protein in jejunum and ***,the expression levels of critical inflammation molecules(interleukin-1β,interleukin-6,tumor necrosis factor-α,and NF-κB)were downregulated(P0.05)by embelin in jejunal and ileal ***,the activity of PCAF were down-regulated(P0.05)by ***,transfection of PCAF siRNAs to IPEC-1 cell decreased NF-κB activities;embelin treatment downregulated(P0.05)the acetylation and activities of NF-κB by 31.7%-74.6%in IPEC-1 cells with overexpression of ***:These results suggested that embelin ameliorates intestinal inflammation in weaned pigs,which might be mediated by suppressing the PCAF/NF-κB signaling pathway.