Effect of fatty acids on poly-amine contents and Na^+/H^+ antiport activity in PM vesicles isolated from roots of barley under salt stress

作     者：Zhao, FG Shu, HR 

作者机构：Shandong Agricultural Univ Sch Hort Tai An 271018 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：2002年第47卷第14期

页      面：1187-1191页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：We are grateful to Prof. Huang Huacheng (School of Academic Science, Shandong Agricultural University) for his useful suggestions This work is a part of post doctoral work of the author 

主　　题：fatty acids plasma membrane vesicles polyamines Na+/H+ antiport barley. 

摘      要：With 200 mmol/L NaCl treatment on barley cultivar Jian 4 (Hordeum vulgare L. cv. J4) seedlings for 6 d, the contents of covalently and noncovalently conjugated polyamines (PAs) and activities of H+-ATPase in plasma membrane (PM) vesicles isolated from the roots decreased remarkably. Moreover, the activity of Na+/H+ antiport was detected first in PM vesicles. The results showed that the decrease in the contents of membrane phospholipid, noncovalently conjugated PAs and activity of H+-ATPase caused by NaCl could be restored partially by application of 1 mmol/L stearic acid (C16:0) and linoleic acid (C18:2), and C18:2 was more effective than C16:0. In addition, a reduction in the contents of covalently conjugated PAs was only reversed partially in the presence of C18:2. Furthermore, Na+/H+ antiport activity was strengthened by exogenous C16:0 and C18:2, and C18:2 was more effective than C16:0. The correlative analysis suggested that, after application of C16:0 and C18:2 under salt stress, there was a