Huangqi Decoction,a compound Chinese herbal medicine,inhibits the proliferation and activation of hepatic stellate cells by regulating the long noncoding RNA-C18orf26-1/microRNA-663a/transforming growth factor-βaxis

作     者：Ben-sheng Dong Fu-qun Liu Wen-na Yang Xiao-dong Li Miao-juan Shi Mao-rong Li Xiu-li Yan Hui Zhang Ben-sheng Dong;Fu-qun Liu;Wen-na Yang;Xiao-dong Li;Miao-juan Shi;Mao-rong Li;Xiu-li Yan;Hui Zhang

作者机构：Traditional Chinese Medicine Epigenomics Research CenterInstitute of Interdisciplinary Integrative Medicine ResearchShanghai University of Traditional Chinese MedicineShanghai 201203China Department of Rheumatology and ImmunologyNanjing Lishui District Hospital of Traditional Chinese MedicineNanjing 211299Jiangsu ProvinceChina Department of Rheumatology and ImmunologyYangzhou University Medical CollegeYangzhou 225000Jiangsu ProvinceChina Department of Cardiovascular PhysiologyFaculty of MedicineKagawa UniversityKita-gunKagawa 761-0793Japan Department of GastroenterologyYueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese MedicineShanghai 200437China 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2023年第21卷第1期

页      面：47-61页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by grants from the National Natural Science Foundation of China (No.82074101 and No.81773979) Shanghai Municipal Health Commission (No.202040486) 

主　　题：Longnoncoding RNA-C18orf26-1 MicroRNA-663a Transforming growth factor-β Hepatic stellate cells Huangqi Decoction 

摘      要：Objective Huangqi Decoction(HQD),a classical traditional Chinese medicine formula,has been used as a valid treatment for alleviating liver fibrosis;however,the underlying molecular mechanism is still *** our previous studies showed that microRNA-663a(miR-663a)suppresses the proliferation and activation of hepatic stellate cells(HSCs)and the transforming growth factor-β/small mothers against decapentaplegic(TGF-β/Smad)pathway,whether long noncoding RNAs(lncRNAs)are involved in HSC activation via the miR-663a/TGF-β/Smad signaling pathway has not yet *** present study aimed to investigate the roles of lncRNA lnc-C18orf26-1 in the activation of HSCs and the mechanism by which HQD inhibits hepatic *** The expression levels of lnc-C18orf26-1,miR-663a and related genes were measured by quantitative reverse transcription-polymerase chain *** were transfected with the miR-663a mimic or inhibitor and lnc-C18orf26-1 small interfering *** water-soluble tetrazolium salt-1 assay was used to assess the proliferation rate of *** in lncRNA expression were evaluated in miR-663a-overexpressing HSCs by using microarray to identify miR-663a-regulated *** hybrid was used to predict the potential miR-663a binding sites on *** reporter assays further confirmed the interaction between miR-663a and the *** expression levels of collagen α-2(I)chain(COL1A2),α-smooth muscle actin(α-SMA)and TGF-β/Smad signaling pathway-related proteins were determined using Western *** Lnc-C18orf26-1 was upregulated in TGF-β1-activated HSCs and competitively bound to *** of lnc-C18orf26-1 inhibited HSC proliferation and activation,downregulated TGF-β1-stimulatedα-SMA and COL1A2 expression,and inhibited the TGF-β1/Smad signaling *** suppressed the proliferation and activation of *** increased miR-663a expression and decreased lnc-C18orf26-1 expression in *** studies showed tha