An efficient CRISPR/Cas9 platform for targeted genome editing in rose(Rosa hybrida)

作     者：Chengpeng Wang Yang Li Na Wang Qin Yu Yonghong Li Junping Gao Xiaofeng Zhou Nan Ma Chengpeng Wang;Yang Li;Na Wang;Qin Yu;Yonghong Li;Junping Gao;Xiaofeng Zhou;Nan Ma

作者机构：Beijing Key Laboratory of Development and Quality Control of Ornamental CropsDepartment of Ornamental HorticultureChina Agricultural UniversityBeijing 100193China School of Applied Chemistry and BiotechnologyShenzhen PolytechnicShenzhen 518038China Key Laboratory of East China Urban AgricultureMinistry of Agriculture and Rural AffairsInstitute of Leisure AgricultureShandong Academy of Agricultural SciencesJinan 250100China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第4期

页      面：895-899页

核心收录：

学科分类：0710[理学-生物学] 090706[农学-园林植物与观赏园艺] 0907[农学-林学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：supported by the National Natural Science Foundation of China(31972438 and 32102427) the Natural Science Foundation of Shandong Province(ZR2021QC130) the Construction of Beijing Science and Technology Innovation and Service Capacity in Top Subjects(CEFF-PXM2019_014207_000032) 

主　　题：CRISPR/CAS9 gene editing RhEIN2 rose 

摘      要：The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-related nuclease 9(Cas9)system enables precise,simple editing of genes in many animals and ***,this system has not been applied to rose(Rosa hybrida)due to the genomic complexity and lack of an efficient transformation technology for this ***,we established a platform for screening single-guide RNAs(sgRNAs)with high editing efficiency for CRISPR/Cas9-mediated gene editing in rose using suspension *** used the Arabidopsis thaliana U6-29 promoter,which showed high activity for driving sgRNA expression,to modify the CRISPR/Cas9 *** used our highly efficient optimized CRISPR/Cas9 system to successfully edit RhEIN2,encoding an indispensable component of the ethylene signaling pathway,resulting in ethylene insensitivity in *** optimized CRISPR/Cas9 system provides a powerful toolbox for functional genomics,molecular breeding,and synthetic biology in rose.