Expression and functional characterization of odorant-binding protein 2 in the predatory mite Neoseiulus barkeri
作者机构：Key Laboratory of Entomology and Pest Control EngineeringCollege of Plant ProtectionSouthwest UniversityBeibei DistrictChongqing400715 China
出 版 物：《Insect Science》 (昆虫科学（英文版）)
年 卷 期：2023年第30卷第5期
页 面：1493-1506页
核心收录：
学科分类：0710[理学-生物学] 07[理学] 09[农学]
基 金：This research was funded by the National Key R&D Program of China(2021YFC2600100) the National Natural Science Foundation of China(32072483,31901944) the Natural Science Foundation of Chongqing,China(cstc2020jcyj-msxmX0407) the Technology Innovation and Application Development Foundation of Chongqing,China(cstc2021jscx-lyjsAX0005) the Fundamental Research Funds for the Central Universities(SWUKQ22019)
主 题：binding affinity crawling speed heterologous expression methyl salicylate olfactory behavior RNA interference
摘 要：Olfaction plays a crucial role for arthropods in foraging,mating,and *** odorant-binding protein(OBP)gene is considered one of the most important olfactory ***,little is known about its functions in predatory ***,we used Neoseiulus barkeri,an important commercialized natural pest control,to explore the chemosensory characteristics of *** this study,*** was attracted by methyl salicylate(MeSA)and showed higher crawling speeds under MeSA ***,we identified and cloned an OBP gene named Nbarobp2 and analyzed its expression profiles in the predatory ***2 was 663 bp,was highly expressed in larval and nymphal stages,and was significantly upregulated in *** under MeSA ***2 encoded 202 amino acid residues with a molecular weight of 23 kDa(after removing the signal peptide).Sequence comparisons revealed that the OBPs in Arachnida shared 6 conserved cysteine sites,but were distinguishable from the OBPs of Insecta on the phylogenetic *** interference,Western blotting,and binding affinity assays further proved that Nbarobp2 was involved in volatile perception in predatory *** study shed light on the functional characteristics of OBPs in predatory mites,providing a new insight for better biological control.
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