Development and Characterization of 35 SNP Markers in the Fat Greenling Hexagrammos otakii
Development and Characterization of 35 SNP Markers in the Fat Greenling Hexagrammos otakii作者机构：Liaoning Key Laboratory of Marine Fishery Molecular Biology Liaoning Ocean and Fisheries Science Institute Dalian China Dalian Eco-Environmental Monitoring Center of Liaoning Province Dalian China
出 版 物：《Open Journal of Marine Science》 (海洋科学期刊（英文）)
年 卷 期：2023年第13卷第1期
页 面：1-6页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Hexagrammos otakii SNP RAD Genetic Diversity
摘 要：Hexagrammos otakii is an important kind of economic fish species in East Asia. However, wild H. otakii resources have declined sharply in recent years as a result of human disturbance and habitat destruction. Thus, it is crucial to protect the current resources of H. otakii. In this study, 35 novel single nucleotide polymorphism (SNP) markers were developed based on restriction-site associated DNA sequencing. The results showed that the observed heterozygosity and expected heterozygosity ranged from 0.1875 to 0.6562 and 0.2679 to 0.5079, respectively. The minor allele frequency ranged from 0.1875 to 0.4375. Polymorphic information content ranged from 0.229 to 0.375. Six SNPs were found to be deviated significantly from the HWE (P H. otakii.
维普期刊数据库