GhAP1-D3 positively regulates flowering time and early maturity with no yield and fiber quality penalties in upland cotton

作     者：Caixiang Wang Juanjuan Liu Xiaoyu Xie Ji Wang Qi Ma Pengyun Chen Delong Yang Xiongfeng Ma Fushun Hao Junji Su Caixiang Wang;Juanjuan Liu;Xiaoyu Xie;Ji Wang;Qi Ma;Pengyun Chen;Delong Yang;Xiongfeng Ma;Fushun Hao;Junji Su

作者机构：State Key Laboratory of Aridland Crop ScienceCollege of Life Science and TechnologyGansu Agricultural UniversityLanzhou 730070China State Key Laboratory of Cotton BiologyCollege of Life ScienceHenan UniversityKaifeng 475004China Cotton Research InstituteXinjiang Academy of Agricultural and Reclamation ScienceShihezi 832000China State Key Laboratory of Cotton BiologyInstitute of Cotton Research of Chinese Academy of Agricultural SciencesAnyang 455000China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第4期

页      面：985-1002页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：funded by the National Natural Science Foundation of China(31971986 and 32260478) the Education Technology Innovation Project of Gansu Province(2022QB-076) the Gansu Province Science and Technology Program(20JR10RA520) the Biological Breeding program of Gansu Academy of Agricultural Sciences(2022GAAS04) the Tianshan talent plan of Xinjiang Autonomous Region(2021) 

主　　题：upland cotton early maturity genome‐wide association studies QTL AP1 flowering time 

摘      要：Flowering time(FTi)is a major factor determining how quickly cotton plants reach *** maturity greatly affects lint yield and fiber quality and is crucial for mechanical harvesting of cotton in northwestern ***,few quantitative trait loci(QTLs)or genes regulating early maturity have been reported in cotton,and the underlying regulatory mechanisms are largely *** this study,we characterized 152,68,and 101 loci that were significantly associated with the three key early maturity traits—FTi,flower and boll period(FBP)and whole growth period(WGP),respectively,via four genome-wide association study methods in upland cotton(Gossypium hirsutum).We focused on one major early maturity-related genomic region containing three single nucleotide polymorphisms on chromosome D03,and determined that GhAP1-D3,a gene homologous to Arabidopsis thaliana APETALA1(AP1),is the causal locus in this *** plants overexpressing GhAP1-D3 showed significantly early flowering and early maturity without penalties for yield and fiber quality compared to wild-type(WT)*** contrast,the mutant lines of GhAP1-D3 generated by genome editing displayed markedly later flowering than the ***1-D3 interacted with GhSOC1(SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1),a pivotal regulator of FTi,both in vitro and in *** in GhAP1-D3 transcript levels clearly affected the expression of multiple key flowering regulatory ***,DNA hypomethylation and high levels of H3K9ac affected strong expression of GhAP1-D3 in early-maturing cotton *** propose that epigenetic modifications modulate GhAP1-D3 expression to positively regulate FTi in cotton through interaction of the encoded GhAP1 with GhSOC1 and affecting the transcription of multiple flowering-related *** findings may also lay a foundation for breeding early-maturing cotton varieties in the future.