Variations in OsSPL10 confer drought tolerance by directly regulating OsNAC2 expression and ROS production in rice

作     者：Yingxiu Li Shichen Han Xingming Sun Najeeb Ullah Khan Qun Zhong Zhanying Zhang Hongliang Zhang Feng Ming Zichao Li Jinjie Li Yingxiu Li;Shichen Han;Xingming Sun;Najeeb Ullah Khan;Qun Zhong;Zhanying Zhang;Hongliang Zhang;Feng Ming;Zichao Li;Jinjie Li

作者机构：State Key Laboratory of Agrobiotechnology/Beijing Key Laboratory of Crop Genetic ImprovementCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Shanghai Key Laboratory of Plant Molecular SciencesCollege of Life SciencesShanghai Normal UniversityShanghai 200234China Sanya Institute of China Agricultural UniversitySanya 572025China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第4期

页      面：918-933页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by grants from the National Natural Science Foundation of China(31861143007) the National Key R&D Program(2021YFD1200502) the Research Program of Sanya Yazhou Bay Science and Technology City(SYND-2022-16) the Project of Hainan Yazhou Bay Seed Lab(B21HJ0508)。 

主　　题：drought tolerance haplotype OsNAC2 OsSPL10 ROS 

摘      要：Drought is a major factor restricting the production of rice(Oryza sativa L.).The identification of natural variants for drought stress-related genes is an important step toward developing genetically improved rice varieties.Here,we characterized a member of the SQUAMOSA PROMOTER BINDING PROTEIN-LIKE(SPL)family,OsSPL10,as a transcription factor involved in the regulation of drought tolerance in rice.OsSPL10 appears to play a vital role in drought tolerance by controlling reactive oxygen species(ROS)production and stomatal movements.Haplotype and allele frequency analyses of OsSPL10 indicated that most upland rice and improved lowland rice varieties harbor the OsSPL10Hap1allele,whereas the OsSPL10Hap2allele was mainly present in lowland and landrace rice varieties.Importantly,we demonstrated that the varieties with the OsSPL10Hap1allele showed low expression levels of OsSPL10 and its downstream gene,OsNAC2,which decreases the expression of OsAP37 and increases the expression of OsCOX11,thus preventing ROS accumulation and programmed cell death(PCD).Furthermore,the knockdown or knockout of OsSPL10 induced fast stomatal closure and prevented water loss,thereby improving drought tolerance in rice.Based on these observations,we propose that OsSPL10 confers drought tolerance by regulating OsNAC2 expression and that OsSPL10Hap1could be a valuable haplotype for the genetic improvement of drought tolerance in rice.