Rapid identification of stigmastane-type steroid saponins from Vernonia amygdalina leaf based onα-glucosidase inhibiting activity and molecular networking
作者机构：School of Food Science and Pharmaceutical EngineeringTesting&Analysis CenterNanjing Normal UniversityNanjing 210023China Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural MedicinesChina Pharmaceutical UniversityNanjing 210009China
出 版 物：《Chinese Journal of Natural Medicines》 (中国天然药物（英文版）)
年 卷 期：2022年第20卷第11期
页 面：846-853页
核心收录：
学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学]
基 金：supported by the National Natural Science Foundation of China(No.81573550)
主 题：Δ^(7,9(11))Stigmastane-type steroid saponins α-Glucosidase inhibitory activity Fragmentation pathways Diagnostic ions Molecular networking
摘 要：Steroid saponins are secondary metabolites with multiple medicinal values that are found in large quantities in natural medicines,especially Vernonia amygdalina,a famous nature medicine for the treatment of tonsillitis,diabetes,*** current study was designed to combine molecular networking(MN)with diagnostic ions for rapid identification ofΔ^(7,9(11))stigmastane-type saponins which were theα-glucosidase inhibitory active substances in ***,theα-glucosidase inhibitory activities of fiveΔ^(7,9(11))stigmastane-type steroid saponins that were previously isolated were screened,which indicated that theΔ^(7,9(11))stigmastane-type steroid saponin was one of the active constituents responsible for ameliorating ***,a strategy was proposed to identify stigmastane-type steroid saponins and verify the plausibility of derived fragmentation pathways by applying MN,MolNetEnhancer and unsupervised substructure annotation(MS2LDA).Based on this strategy,other sevenΔ^(7,9(11))stigmastane-type steroid saponins were identified from this *** research provide scientific evidence for the antidiabetic potential of the steroid saponin-rich extract of *** leaf.
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