Enrichment and analysis of circulating tumor cells by integrating multivalent membrane nano-interface and endogenous enzyme-signal amplification

作     者：Mengjiao Wang Dayong Li Chengjie Duan Jin Jiao Youjing Gong Xiaoping Wang Zhongyun Wang Yang Xiang Mengjiao Wang;Dayong Li;Chengjie Duan;Jin Jiao;Youjing Gong;Xiaoping Wang;Zhongyun Wang;Yang Xiang

作者机构：State Key Laboratory of Pharmaceutical BiotechnologySchool of Life SciencesNanjing UniversityNanjing 210023China State Key Laboratory of Natural and Biomimetic DrugsPeking UniversityBeijing 100191China Department of NeurologyTongren HospitalShanghai JiaoTong University School of MedicineShanghai 200336China Department of AnesthesiologyThe First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2023年第34卷第1期

页      面：574-577页

核心收录：

学科分类：1002[医学-临床医学] 0703[理学-化学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.81672570) the State Key Laboratory of Natural and Biomimetic Drugs(No.K202009) 

主　　题：CTCs Nano-interface Alkaline phosphatase dimer Signal amplification Liposomes 

摘      要：For circulating tumor cells(CTCs)-based cancer diagnosis and monitoring,effective enrichment and specific analysis of CTCs present significant *** biomembrane interfaces can enhance the highaffinity interactions between various receptors and ligands in life activities by mediating the rearrangement and positioning of membrane-bound components through its *** by this,we have constructed a multivalent membrane nano-interface using aptamer-linked liposomes for the efficient capture of ***,the subsequent introduction of rolling circle amplification(RCA)reaction has increased the number of aptamers and extended them to the surrounding space to improve the affinity of the membrane nano-interface for *** CTCs are enriched,alkaline phosphatase overexpressed on the surface of tumor cells is used as endogenous enzyme-mediated signal amplification by catalyzing 4-nitrophenyl phosphate(p NPP)with color change,achieving the analysis of ***,the enrichment and visual analysis of human hepatocellular carcinoma(HepG2)with a detection limit of 10 cells/m L can be obtained by integrating the multivalent membrane nano-interface and endogenous enzyme signal *** detection of the target in the serum proved this method has the potential for further clinical application and provides a potential method for studying the correlation between alkaline phosphatase dimer and cancer progression.