Exosome-transported IncRNA H19 regulates insulin-like growth factor-1 via the H19/let-7a/insulin-like growth factor-1 receptor axis in ischemic stroke

作     者：Jue Wang Bin Cao Yan Gao Yu-Hua Chen Juan Feng Jue Wang;Bin Cao;Yan Gao;Yu-Hua Chen;Juan Feng

作者机构：Department of NeurologyShengjing Hospital of China Medical UniversityShenyangLiaoning ProvinceChina Department of Developmental Cell BiologyKey Laboratory of Cell BiologyMinistry of Public Healthand Key Laboratory of Medical Cell BiologyMinistry of EducationChina Medical UniversityShenyangLiaoning ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2023年第18卷第6期

页      面：1316-1320页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,No.82271353(to JW) Key Research and Development Program of Liaoning Province,No.2020JH2/10300047(to JF) 

主　　题：cerebral ischemia exosomes H19 insulin-like growth factor-1 insulin-like growth factor 1 receptor ischemic stroke long non-coding RNA 

摘      要：LncRNA(long non-coding RNA) H19 is a transcript of the H19 gene that is expressed during *** previously discove red a role for circular lncRNA H19 in the onset and prognosis of cerebral ischemic *** this study,we used serum from patients with ischemic stroke,and mouse and cell culture models to elucidate the roles of plasma and neuronal exosomes in the regulatory effect of lncRNA H19 on insulin-like growth factor-1 and its mechanism in ischemic stroke,using western blotting,quantitative real-time polymerase chain reaction,and enzyme-linked immunosorbent *** exosomal IncRNA H19 was negatively associated with blood levels of insulin-like growth factor-1 in samples from patients with cerebral ischemic *** a mouse model,levels of exosomal IncRNA H19 were positively correlated with plasma and cerebral lncRNA *** a cell co-culture model,we confirmed that IncRNA H19 was transported from neuro ns to astrocytes by exosomes to induce downregulation of insulin-like growth factor-1 through the H19/let-7 a/insulin-like growth factor-1 receptor *** study provides the first evidence for the transpo rtation of IncRNA H19 by exosomes and the relationship between IncRNA H19 and insulinlike growth factor-1.