Embryo Rescue via Artificial Seed Technique and Long-Term Preservation of Zephyranthes
Embryo Rescue via Artificial Seed Technique and Long-Term Preservation of Zephyranthes作者机构：Department of Earth and Environment Institute of Environment Florida International University Miami USA Fairchild Tropical Botanic Garden Coral Gables USA Department of Horticulture and Landscape Architecture Oklahoma State University Stillwater USA
出 版 物：《American Journal of Plant Sciences》 (美国植物学期刊（英文）)
年 卷 期：2022年第13卷第11期
页 面：1347-1359页
学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学]
主 题：Rain Lily Synthetic Seed Cryopreservation Encapsulation Ornamental Landscape Zephyranthes atamasc Zephyranthes grandiflora
摘 要：Zephyranthes is valued as a native ornamental landscaping plant and a traditional medicinal herb. Due to the low seed viability, this study was carried out to evaluate the potential of seed embryo rescue using the artificial seed technique and long-preservation in Z. atamasca and Z. grandiflora. Seed embryos were selected for encapsulation with different concentration of sodium alginate (3%, 4%, and 5%) and calcium chloride (either 25, 50, 75, and 100 mM) followed by no encapsulated embryos as a control. The greatest viability of encapsulated embryos was 95% in Z. grandiflora and 85% in Z. atamasca with the combination of 4% sodium alginate and 100 mM calcium chloride after two weeks at 5°C. The highest viability with A490nm0.12 and A490nm0.16 were achieved when embryos were cultured in pretreatment medium with 30 g/L sucrose in Z. grandiflora and Z. atamasca, respectively. The highest viability by TTC assay after cryopreservation was observed with 54% viability for Z. grandiflora and 48% viability with Z. atamasca, after 2 h of dehydration. Rain lilies embryos were successfully preserved functioned as artificial seed and cryopreservation using encapsulation-dehydration method has been established for both species that can be used for other flower species with some modifications.
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