A zwitterionic polymer-inspired material mediated efficient CRISPR-Cas9 gene editing

作     者：Lingmin Zhang Langyu Yang Jionghua Huang Sheng Chen Chuangjia Huang Yinshan Lin Ao Shen ZhouYikang Zheng Wenfu Zheng Shunqing Tang Lingmin Zhang;Langyu Yang;Jionghua Huang;Sheng Chen;Chuangjia Huang;Yinshan Lin;Ao Shen;ZhouYikang Zheng;Wenfu Zheng;Shunqing Tang

作者机构：Key Laboratory of Molecular Target&Clinical Pharmacology and the State&NMPA Key Laboratory of Respiratory DiseaseSchool of Pharmaceutical Sciences&The Third and Fifth Affiliated HospitalGuangzhou Medical UniversityGuangzhou 511436China Department of Biomedical EngineeringJinan UniversityGuangzhou 510632China CAS Key Lab for Biological Effects of Nanomaterials and NanosafetyNational Center for NanoScience and TechnologyBeijing 100190China 

出 版 物：《Asian Journal of Pharmaceutical Sciences》 (亚洲药物制剂科学（英文）)

年 卷 期：2022年第17卷第5期

页      面：666-678页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学] 

基　　金：National Natural Science Foundation of China(82072047,81700382) Natural Science Foundation of Guangdong Province(2019A1515012166) Research Foundation of Education Bureau of Guangdong Province(2021ZDZX2004) Basic and Applied Basic Research Project of Guangzhou(02080390) Outstanding Youth Development Program of Guangzhou Medical University 

主　　题：CRISPR/Cas9 Gene editing Zwitterionic polymers CD44 PLK1 

摘      要：The typeⅡ prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR/Cas9) adaptive immune system is a cutting-edge genome-editing ***,its applications are still limited by its inefficient ***,we present a novel gene vector,the zwitterionic polymer-inspired material with branched structure (ZEBRA) for efficient CRISPR/Cas9 ***-like kinase 1 (PLK1) acts as a master regulator of mitosis and overexpresses in multiple tumor *** Cas9 and single guide sgRNA (sgRNA)-encoded plasmid was transduced to knockout Plk1 gene,which was expected to inhibit the expression of *** studies demonstrated that ZEBRA enabled to transduce the CRISPR/Cas9 system with large size into the cells *** transduction with ZEBRA was cell line dependent,which showed~10-fold higher in CD44-positive cancer cell lines compared with CD44-negative ***,ZEBRA induced highlevel expression of Cas9 proteins by the delivery of CRISPR/Cas9 and efficient gene editing of Plk1 gene,and inhibited the tumor cell growth *** zwitterionic polymerinspired material is an effective and targeted gene delivery vector and further studies are required to explore its potential in gene delivery applications.