Expression of the methylcytosine dioxygenase ten-eleven translocation-2 and connexin 43 in inflammatory bowel disease and colorectal cancer

作     者：Mohammad El-Harakeh Jessica Saliba Kawthar Sharaf Aldeen May Haidar Layal El Hajjar Mireille Kallassy Awad Jana G Hashash Margret Shirinian Marwan El-Sabban 

作者机构：Department of AnatomyCell Biology and Physiological SciencesFaculty of MedicineAmerican University of BeirutBeirut 1107Lebanon UR GPF Laboratory of Biodiversity and Functional GenomicsFaculty of ScienceUniversitéSaint-Joseph de BeyrouthBeirut 1107Lebanon Department of BiologyFaculty of SciencesLebanese UniversityBeirut 1533Lebanon Department of Public HealthFaculty of Health SciencesUniversity of BalamandDekwanehSin el Fil 1552Lebanon Department of Internal MedicineDivision of Gastroenterology and HepatologyFaculty of MedicineAmerican University of BeirutBeirut 1107Lebanon Department of Experimental PathologyImmunology and MicrobiologyFaculty of MedicineAmerican University of BeirutBeirut 1107Lebanon 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2022年第28卷第40期

页      面：5845-5864页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Demethylation Inflammation-induced carcinogenesis Ulcerative colitis Colorectal cancer Connexins 

摘      要：BACKGROUND Inflammatory bowel disease(IBD)constitutes a substantial risk factor for colorectal *** 43(Cx43)is a protein that forms gap junction(GJ)complexes involved in intercellular communication,and its expression is altered under pathological conditions,such as IBD and *** studies have implicated epigenetic processes modulating DNA methylation in the pathogenesis of diverse inflammatory and malignant *** ten-eleven translocation-2(TET-2)enzyme catalyzes the demethylation,hence,regulating the activity of various cancer-promoting and tumor-suppressor *** To investigate Cx43 and TET-2 expression levels and presence of 5-hydroxymethylcytosine(5-hmC)marks under inflammatory conditions both in vitro and in *** TET-2 expression was evaluated in parental HT-29 cells and in HT-29 cells expressing low or high levels of Cx43,a putative tumor-suppressor gene whose expression varies in IBD and colorectal cancer,and which has been implicated in the inflammatory process and in tumor *** dextran sulfate sodium-induced colitis model was reproduced in BALB/c mice to evaluate the expression of TET-2 and Cx43 under inflammatory conditions in *** addition,archived colon tissue sections from normal,IBD(ulcerative colitis),and sporadic colon adenocarcinoma patients were obtained and evaluated for the expression of TET-2 and *** levels were reported at the transcriptional level by quantitative real-time polymerase chain reaction,and at the translational level by Western blotting and *** Under inflammatory conditions,Cx43 and TET-2 expression levels increased compared to noninflammatory ***-2 upregulation was more pronounced in Cx43-deficient ***,colon tissue sections from normal,ulcerative colitis,and sporadic colon adenocarcinoma patients corroborated that Cx43 expression increased in IBD and decreased in adenocarcinoma,compared to tissues from non-IBD ***,TET-2