Salicylic acid biosynthesis is not from phenylalanine in Arabidopsis

作     者：Jie Wu Wentao Zhu Qiao Zhao Jie Wu;Wentao Zhu;Qiao Zhao

作者机构：Shenzhen Key Laboratory of Synthetic GenomicsGuangdong Provincial Key Laboratory of Synthetic GenomicsShenzhen Institute of Synthetic BiologyShenzhen Institute of Advanced Technologythe Chinese Academy of SciencesShenzhen 518055China Department of Applied ChemistryInnovation Center of Pesticide ResearchCollege of ScienceChina Agricultural UniversityBeijing 100193China Center for Plant BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第4期

页      面：881-887页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：supported by the National Key Research and Development Program of China(2021YFD2201000,2019YFA0903900) National Natural Science Foundation of China(31870273) Shenzhen Key Laboratory of Synthetic Genomics(ZDSYS201802061806209) The Strategic Priority Research Program of the Chinese Academy of Sciences(XDPB18) 

主　　题：benzoic acid IC pathway isotopic labeling PAL pathway salicylic acid 

摘      要：The phytohormone salicylic acid(SA)regulates biotic and abiotic stress responses in *** distinct biosynthetic pathways for SA have been well documented in plants:the isochorismate(IC)pathway in the chloroplast and the phenylalanine ammonia-lyase(PAL)pathway in the ***,there has been no solid evidence that the PAL pathway contributes to SA ***,we report that feeding Arabidopsis thaliana with Ring-13C-labeled phenylalanine(13C6-Phe)resulted in incorporation of the13C label not into SA,but into its isomer 4-hydroxybenzoic acid(4-HBA)*** obtained similar results when feeding13C6-Phe to the SA-deficient ics1 ics2 mutant and the SA-hyperaccumulating mutant s3h ***,we detected13C6-SA when13C6-benzoic acid(BA)was provided,suggesting that SA can be synthesized from ***,despite the substantial accumulation of SA upon pathogen infection,we did not observe incorporation of13C label from Phe into *** also did not detect13C6-SA in PAL-overexpressing lines in the kfb01 kfb02 kfb39 kfb50 background after being fed13C6-Phe,although endogenous PAL levels were dramatically *** on these combined results,we propose that SA biosynthesis is not from Phe in *** results have important implications for our understanding of the SA biosynthetic pathway in land plants.