Dietary ellagic acid supplementation attenuates intestinal damage and oxidative stress by regulating gut microbiota in weanling piglets

作     者：Wenxia Qin Baoyang Xu Yuwen Chen Wenbo Yang Yunzheng Xu Juncheng Huang Ting Duo Yihua Mao Guozong Zhou Xianghua Yan Libao Ma Wenxia Qin;Baoyang Xu;Yuwen Chen;Wenbo Yang;Yunzheng Xu;Juncheng Huang;Ting Duo;Yihua Mao;Guozong Zhou;Xianghua Yan;Libao Ma

作者机构：State Key Laboratory of Agricultural MicrobiologyCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhanChina The Cooperative Innovation Center for Sustainable Pig ProductionWuhanChina Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety TechnologyWuhanChina Wuhan Huayang Animal Pharmaceutical Co.Ltd WuhanChina Hubei Tianxin Biotech Co.LtdShiyanChina 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2022年第11卷第4期

页      面：322-333页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported by the National Natural Science Foundation Regional Innovation and Development Joint Fund Project(U20A2055) Agricultural Microbiology of Large Research Infrastructures(463119009) 

主　　题：Ellagic acid Gut microbiota Weanling piglets Intestinal damage Oxidative stress 

摘      要：Intestinal oxidative stress triggers gut microbiota dysbiosis,which is involved in the etiology of postweaning diarrhea and enteric *** acid(EA)can potentially serve as an antioxidant supplement to facilitate weaning transition by improving intestinal oxidative stress and gut microbiota ***,we aimed to investigate the effects of dietary EA supplementation on the attenuation of intestinal damage,oxidative stress,and dysbiosis of gut microbiota in weanling piglets.A total of126 piglets were randomly assigned into 3 groups and treated with a basal diet and 2 m L saline orally(Ctrl group),or the basal diet supplemented with 0.1%EA and 2 m L saline orally(EA group),or the basal diet and 2 m L fecal microbiota suspension from the EA group orally(FEA group),respectively,for 14 *** with the Ctrl group,EA group improved growth performance by increasing average daily feed intake and average daily weight gain(P0.05)and decreasing fecal scores(P0.05).EA group also alleviated intestinal damage by increasing the tight junction protein occludin(P0.05),villus height,and villus height-to-crypt depth ratio(P0.05),while decreasing intestinal epithelial apoptosis(P0.05).Additionally,EA group enhanced the jejunum antioxidant capacity by increasing the total antioxidant capacity(P0.01),catalase(P0.05),and glutathione/oxidized glutathione(P0.05),but decreased the oxidative metabolite malondialdehyde(P0.05)compared to the Ctrl *** with the Ctrl group,EA and FEA groups increased alpha diversity(P0.05),enriched beneficial bacteria(Ruminococcaceae and Clostridium ramosum),and increased metabolites short-chain fatty acids(P0.05).Correspondingly,FEA group gained effects comparable to those of EA group on growth performance,intestinal damage,and intestinal antioxidant *** addition,the relative abundance of bacteria shifted in EA and FEA groups was significantly related to the examined indices(P0.05).Overall,dietary EA supplemen