The clock gene,period,influences migratory flight and reproduction of the oriental armyworm,Mythimna separata(Walker)

作     者：Jiayue Ji Yueqiu Liu Lei Zhang Yunxia Cheng David Stanley Xingfu Jiang 

作者机构：State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina School of Landscape ArchitectureBejing University of AgricultureBejingChina Biological Control of Insects Research LaboratoryUSDA/Agricultural Research ServiceColumbiaMOUSA 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2023年第30卷第3期

页      面：650-660页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 090402[农学-农业昆虫与害虫防治] 

基　　金：the National Natural Science Foundation of China(No.32072420,31871951,31672019) China Agriculture Research System of MOF and MARA(CARS-22) the National Key Research and Development Program of China(2017YFD0201802,2017YFD0201701) 

主　　题：flight action Mythimna separata period clock gene reproduction RNA interference 

摘      要：The oriental armyworm,Mythimna separata,is a major long-distance migratory insect pest of grain crops in China and other Asian *** flights and reproductive behavior usually occur at night,regulated by a circadian ***,knowledge about the linkages between adult flight,reproduction,and clock genes is still *** fill this important gap in our knowledge,a clock gene(designated Msper)was identified and phylogenetic analysis indicated that the encoded protein(MsPER)was highly similar to PER proteins from other insect *** RT-PCR assays demonstrated that significantly different spatiotemporal and circadian rhythmic accumulations of mRNA encoding MsPER occurred during development under steady 14 h:10 h light:dark *** highest mRNA accumulation occurred in adult antennae and the lowest in *** was expressed rhythmically in adult antennae,relatively less in photophase and more entering *** small interference RNA(siRNA)into adult heads effectively knocked down Msper mRNA levels within 72 *** siRNA-injected adults reduced their evening flight activity significantly and did not exhibit a normal evening peak of flight *** also failed to mate and lay eggs within 72 *** mating behavior was restored to control levels by 72 h post *** infer that Msper is a prominent clock gene that acts in regulating adult migratory flight and mating behaviors of *** of its influence on migration and mating,Msper may be a valuable gene to target for effective management of this migratory insect.