The NAC transcription factor ONAC083 negatively regulates rice immunity against Magnaporthe oryzae by directly activating transcription of the RING-H2 gene OsRFPH2-6

作     者：Yan Bi Hui Wang Xi Yuan Yuqing Yan Dayong Li Fengming Song Yan Bi;Hui Wang;Xi Yuan;Yuqing Yan;Dayong Li;Fengming Song

作者机构：National Key Laboratory for Rice BiologyInstitute of BiotechnologyZhejiang UniversityHangzhou 310058China College of Chemistry and Life SciencesZhejiang Normal UniversityJinhua 321004China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第3期

页      面：854-875页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by grants from the National Natural Science Foundation of China (31871945, 32072403) the National Key Research and Development Program of China (2016YFD0100600) 

主　　题：ACGCAA element immunity Magnaporthe oryzae ONAC083 OsRFPH2-6 rice 

摘      要：NAC transcription factors(TFs) play critical roles in plant immunity by modulating the expression of downstream genes via binding to specific cis-elements in promoters. Here, we report the function and regulatory network of a pathogenand defense phytohormone-inducible NAC TF gene, ONAC083, in rice(Oryza sativa) ***083 localizes to the nucleus and exhibits transcriptional activation activity that depends on its C-terminal region. Knockout of ONAC083enhances rice immunity against Magnaporthe oryzae, strengthening pathogen-induced defense responses, and boosting chitin-induced pattern-triggered immunity(PTI), whereas ONAC083 overexpression has opposite effects. We identified ONAC083-binding sites in the promoters of 82genes, and showed that ONAC083 specifically binds to a conserved element with the core sequence ACGCAA. ONAC083 activated the transcription of the genes OsRFPH2-6, OsTrx1, and OsPUP4 by directly binding to the ACGCAA element. OsRFPH2-6, encoding a RING-H2 protein with an N-terminal transmembrane region and a C-terminal typical RING domain, negatively regulated rice immunity against M. oryzae and chitin-triggered PTI. These data demonstrate that ONAC083 negatively contributes to rice immunity against M. oryzae by directly activating the transcription of OsRFPH2-6 through the ACGCAA element in its promoter. Overall, our study provides new insight into the molecular regulatory network of NAC TFs in rice immunity.