Protective Effect and Mechanism of Kuntai Capsule on Angiotensin II-Induced Hypertension in Ovariectomized Rats

作     者：GE Xiao-fen LI Sha-sha LIU Yan-hua LU Mei-qiu SU Hui-na YANG Xin SUN Xiao-wan GE Xiao-fen;LI Sha-sha;LIU Yan-hua;LU Mei-qiu;SU Hui-na;YANG Xin;SUN Xiao-wan

作者机构：Department of Obstetrics and GynecologyPeking University People's HospitalBeijing(100044)China Department of Obstetrics and GynecologyDongying People's HospitalDongyingShandong Province(257000)China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2023年第29卷第6期

页      面：526-533页

核心收录：

学科分类：1006[医学-中西医结合] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：Supported by 2020 Bethune Excellent Talent Training Program for Young and Middle-Aged Doctors。 

主　　题：Kuntai Capsule Chinese medicine perimenopausal hypertension angiotensin II 

摘      要：Objective To explore the protective effect and mechanism of Kuntai(KT)Capsule on angiotensin II(Ang II)-induced hypertension in ovariectomized(OVX)rats.Methods Fifty-four rats were randomly divided into 6 groups according to a random number table,9 in each group:control,OVX sham+Ang II,OVX,OVX+Ang II,OVX+Ang II+E2,and OVX+Ang II+KT.OVX rats model was constructed by retroperitoneal bilateral ovariectomy.After 4 weeks of pretreatment with KT Capsule[0.8 g/(kg·d)and 17-β-estradiol(E2,1.2 mg/(kg·d)]respectively,Ang II was injected into a micro-osmotic pump with a syringe to establish a hypertensive rat model.Blood pressure of rat tail artery was measured in a wake state of rats using a non-invasive sphygmomanometer.Blood pressure changes were compared between the intervention groups(OVX+Ang II+KT,OVX+Ang II+E2)and the negative control group(OVX+Ang II).Serum malondialdehyde(MDA)level and superoxide dismutase(SOD)activity were detected respectively.The expressions of oxidative stress-related protein superoxide dismutase2(SOD2)and anti-thioredoxin(TRX),autophagy marker protein[beclin1,light chain(LC)3 II/I ratio and autophagy canonical pathway protein phosphatidylinositol 3-kinase(PI3K)/serine/threonine kinase(AKT)-mammalian target of rapamycin(mTOR)]were evaluated by Western blotting.Results Compared with the OVX+Ang II group,the systolic blood pressure of OVX+Ang II+KT group was significantly lowered(P0.05)but not the diastolic blood pressure.Besides,SOD2 and TRX protein levels in mycardial tissues were significantly reduced in the OVX+Ang II+KT group compared with the OVX+Ang II group(P0.05).Oxidative stress serum markers MDA and SOD were down-and up-regulated in the OVX+Ang II+KT group,respectively(P0.05).Compared with OVX+Ang II group,the levels of cardiac proteins beclin-1 and LC3II/LC3 I in OVX+Ang II+KT group were also up-regulated(P0.05),and the expression levels of p-PI3K,p-AKT and mTOR protein were down-regulated(P0.05).Conclusion KT could protect blood pressure of Ang II-induced OVX rats by inhibiting oxidative stress and up-regulating protective autophagy.