Shikonin alleviates collagen-induced arthritis mice by inhibiting M1 macrophage polarization

作     者：HE Lianhua LUAN Huijie QIN Qingxia HE Juan CHEN Jian HU Yiping CAI Yueming SUN Desheng SHI Yu WANG Qingwen HE Lianhua;LUAN Huijie;QIN Qingxia;HE Juan;CHEN Jian;HU Yiping;CAI Yueming;SUN Desheng;SHI Yu;WANG Qingwen

作者机构：Department of Rheumatism and ImmunologyPeking University Shenzhen HospitalShenzhen 518036China Shenzhen China Resources Sanjiu Pharmaceutical Trading Co.Ltd.Shenzhen 518029China The Key Laboratory of Inflammatory and Immunology DiseasesShenzhen 518036China Department of ultrasoundPeking University Shenzhen HospitalShenzhen 518036China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2022年第42卷第6期

页      面：932-939页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Traditional Chinese Medicine Bureau of Guangdong Province:the Role and Mechanism of Ermiao San in the Regulation of Th cell Differentiation by PKC-? in the Treatment of RA (No. 20211333) National Natural Science Foundation of China:the Role and Mechanism of CD4+T Cell Smad7 Loss in the Pathogenesis of Rheumatoid Arthritis(No. 81974253) the Significance of Regulating Glycolytic Metabolism by HK-2 in Dendritic Cell Migration and RA Pathogenesis (No. 81901641) Shenzhen Science and Technology Innovation Committee:Research on Key Technologies of Intelligent Robot Based on Artificial Intelligence Image Data (No. GJHZ20200731095208025) Municipal Development and Reform Commission:Virtual Reality Clinical Application Public Service Platform (XMHT20190104001) Project of Shenzhen Municipal Health Commission:Biological Markers in Idiopathic Inflammatory Myopathy and Clinical Significance of Tumorigenesis (No. SZXJ2017046) Nature Science Foundation of Guangdong Province:Experimental Study on Treatment of Rabbit Rheumatoid Arthritis with Lipidated Methotrexate Microbubbles Combined with Controlled Release by Ultrasonic Irradiation (No. 2018A030313281) 

主　　题：Shikonin arthritis rheumatoid macrophages M1 polarization 

摘      要：OBJECTIVE: To investigate the effects of shikonin(SKN) on M1 and M2 polarization of macrophages both in vitro and in vivo. METHODS: Collagen-induced arthritis(CIA) in male DBA/1 mice were treated with a dose of 4 mg/kg/day of SKN for 23 d(n = 6/group). The histopathology of inflamed joints in CIA mice was evaluated to test the antiarthritic effect of SKN. M1/M2 polarization of macrophages induced by lipopolysaccharide(LPS) and interferon(IFN)-γ or interleukin(IL)-4 and IL-13, were used to assess the effect of SKN(0.05, 0.1, and 0.2 μM). The effect of SKN on the protein expression of nitric oxide synthase, arginase, CD68, and CD206 was evaluated using western blot analysis. RESULTS: The results of this study revealed that SKN delayed the arthritis feet symptom score, reduced the incidence rate of arthritis, and relieved the inflammation of joints in CIA mice. SKN inhibited M1 macrophage polarization but did not affect M2 macrophage polarization in the joints of CIA mice. Moreover, SKN inhibited M1 polarization induced by LPS and IFN-γ, but did not affect M2 polarization induced by IL-4 and IL-13. CONCLUSION: These findings suggest that SKN alleviated CIA through inhibiting M1 macrophage polarization and has great potential as a new drug for RA treatment.