Evaluation of disinfection byproducts for their ability to affect mitochondrial function
作者机构：Department of Civil and Environmental EngineeringUniversity of NevadaLas VegasNVUSA Department of Environmental and Occupational HealthMilken Institute School of Public HealthGeorge Washington UniversityWashington DCUSA
出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)
年 卷 期：2022年第34卷第7期
页 面：295-304页
核心收录：
学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 0815[工学-水利工程]
基 金：supported by the National Science Foundation (No. 1833108)。
主 题：Disinfection byproducts Toxicity Mitochondrial membrane potential Dose-response curve In vitro bioassay Emerging contaminants
摘 要：In the race to deliver clean water to communities through potable water reuse, disinfection and water quality assessment are and will continue to be fundamental factors. There are over 700 disinfection byproducts(DBPs) in water;evaluating each compound is practically impossible and very time consuming. A bioanalytical approach could be an answer to this challenge. In this work, the response of four major classes of DBPs toward mitochondrial membrane potential( Δψm) and cytoplasmic adenosine triphosphate(C-ATP) was investigated with human carcinoma(Hep G2) cells. Within 90 min of cell exposure, only the haloacetic acid(HAA) mixture caused a cytotoxic response as measured by C-ATP. All four groups(haloacetonitriles(HANs), trihalomethanes(THMs), nitrosamines(NOAs), and HAAs) responded well to Δψm, R 2 0.70. Based on the half-maximum concentration that evoked a 50% response in Δψm, the response gradient was HANs HAAs ~ THM NOAs. The inhibition of the Δψm by HANs is driven by dibromoacetonitrile(DBAN), while dichloroacetonitrile(DCAN) did not cause a significant change in the Δψm at less than 2000 μM. A mixture of HANs exhibited an antagonistic behavior on the Δψm compared to individual compounds. If water samples are concentrated to increase HAN concentrations, especially DBAN, then Δψm could be used as a biomonitoring tool for DBP toxicity.
维普期刊数据库