Role of miRNA-27a in Regulating the Immune Function of Pulmonary Tuberculosis Rat Model

作     者：XU Bai-lan XU Bai-lan

作者机构：Department of Respiratory and Critical Care Medicinethe First Hospital of QuanzhouQuanzhou Fujian 362000China 

出 版 物：《Chinese Journal of Biomedical Engineering(English Edition)》 (中国生物医学工程学报（英文版）)

年 卷 期：2022年第31卷第3期

页      面：112-119,138页

学科分类：1002[医学-临床医学] 10[医学] 

主　　题：miRNA-27a pulmonary tuberculosis rats immune function 

摘      要：Objective:To analyze the role of mi RNA-27a in regulating the immune function of the pulmonary tuberculosis rat model.Methods:40 healthy female rats were divided into 4 groups:healthy control group,model group,silence group,and overexpression group,with 10 rats in each group.The control group was not treated,and the other three groups of rats were injected with 200μL of Mycobacterium bacilli suspension through the tail vein to establish a rat model of pulmonary tuberculosis,in which the model group was not treated,and the silence group was injected with 10μL of mi RNA-27 inhibition vector lentiviral suspension,and the overexpression group was injected with 10μL of mi RNA-27 expression vector lentiviral suspension.The expression level of mi RNA-27a,peripheral blood T lymphocyte subsets,thymus index,inflammatory index,and TLR4/NF-κB signal pathway-related protein level in the four groups of rats were compared and analyzed.Results:Compared with the healthy control group,the expression levels of the silence group and model group were significantly decreased,while the expression level of the overexpression group was significantly increased;Compared with the model group and addiction group,the expression level of the overexpression group was significantly increased,with significant differences(P0.05).Compared with the healthy control group,CD3+,CD4+,CD4+/CD8+,thymus index,IL-10,and IκB in the model group,silence group,and overexpression group were significantly higher than those in the control group;The levels of CD3+,CD4+,CD4+/CD8+,thymus index,IL-10,IκB decreased significantly,with significant differences(P0.05);Compared with the overexpression group,CD8+,IL-6,TNF-α,TLR4,NF-κBp65,and p-IκB levels were significantly lower;The levels of CD3+,CD4+,CD4+/CD8+,thymic index,IL-10,and IκB levels were significantly increased,with significant differences(P0.05).Compared with the silence group,the levels of CD3+,CD4+,CD4+/CD8+,thymic index,IL-10,and IκB were significantly increased and the levels of CD8+,IL-6,TNF-α,TLR4,NF-κBp65,and p-IκB were significantly lower in the overexpression group,with significant differences(P0.05).Conclusion:Overexpression of mi RNA-27a can inhibit the TLR4/NF-κB signal pathway and regulate the immune function of rats with pulmonary tuberculosis,which in turn reduce lung injury in rats.