The Viral Load of Epstein-Barr Virus in Blood of Children after Hematopoietic Stem Cell Transplantation

作     者：WANG Wen Jun FENG Shun Qiao HE Feng DU Hai Jun FENG Miao WANG Rui Fang MEI Guo Yong LIU Mi LIU Rong YAO Hai Lan HAN Jun WANG Wen Jun;FENG Shun Qiao;HE Feng;DU Hai Jun;FENG Miao;WANG Rui Fang;MEI Guo Yong;LIU Mi;LIU Rong;YAO Hai Lan;HAN Jun

作者机构：State Key Laboratory of Infectious Disease Prevention and ControlNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Department of HematologyChildren’s Hospital of Capital Institute of PediatricsBeijing 100020China Department of Biochemistry&ImmunologyCapital Institute of PediatricsBeijing 100020China 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2022年第35卷第9期

页      面：804-810页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：supported by the Research Project Supported by the China Mega-Project for Infectious Disease[2018ZX10102001,2018ZX10711001,and 2018ZX10734404] National Pathogen Resource Collection Center[NPRC-32] the SKLID Development Grant[2011SKLID104]。 

主　　题：Chip digital PCR Epstein-Barr virus Hematopoietic stem cell transplantation Quantitative PCR 

摘      要：Objective To detect the Epstein-Barr virus(EBV)viral load of children after hematopoietic stem cell transplantation(HSCT)using chip digital PCR(cdPCR).Methods The sensitivity of cdPCR was determined using EBV plasmids and the EBV B95-8 strain.The specificity of EBV cdPCR was evaluated using the EBV B95-8 strain and other herpesviruses(herpes simplex virus 1,herpes simplex virus 2,varicella zoster virus,human cytomegalovirus,human herpesvirus 6,and human herpesvirus 7).From May 2019 to September 2020,64 serum samples of children following HSCT were collected.EBV infection and the viral load of serum samples were detected by cdPCR.The epidemiological characteristics of EBV infections were analyzed in HSCT patients.Results The limit of detection of EBV cdPCR was 110 copies/mL,and the limit of detection of EBV quantitative PCR was 327 copies/mL for the pUC57-BALF5 plasmid.The result of EBV cdPCR was up to 121 copies/mL in the EBV B95-8 strain,and both were more sensitive than that of quantitative PCR.Using cdPCR,the incidence of EBV infection was 18.75%in 64 children after HSCT.The minimum EBV viral load was 140 copies/mL,and the maximum viral load was 3,209 copies/mL using cdPCR.The average hospital stay of children with EBV infection(184±91 days)was longer than that of children without EBV infection(125±79 days),P=0.026.Conclusion EBV cdPCR had good sensitivity and specificity.The incidence of EBV infection was 18.75%in 64 children after HSCT from May 2019 to September 2020.EBV cdPCR could therefore be a novel method to detect EBV viral load in children after HSCT.