Identification of Quantitative Trait Loci for Lipid Metabolism in Rice Seeds
Identification of Quantitative Trait Loci for Lipid Metabolism in Rice Seeds作者机构：National Key Laboratory of Plant Molecular Genetics and National Center for Plant Gene Research(Shanghai)Shanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesThe Chinese Academy of SciencesGraduate School of the Chinese Academy of Sciences300 Fenglin RoadShanghai 200032China National Key Laboratory of Rice BiologyChina National Rice Research InstituteHangzhou 310006China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2012年第5卷第4期
页 面：865-875页
核心收录：
学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 0901[农学-作物学]
基 金：This work was supported by grants from the Ministry of Agriculture of China (2009ZX08009--067B 2009ZX08009-102B 2009ZX08001- 022B) the National Natural Science Foundation of China (30730058) and the Shanghai Science and Technology Development (09DJ 1400503)
主 题：Rice fatty acid seed oil lipid metabolism QTL.
摘 要：Plant seed oil is important for human dietary consumption and industrial application. The oil trait is controlled by quantitative trait loci （QTLs）, but no QTLs for fatty acid composition are known in rice, the monocot model plant. QTL analysis was performed using F2 and F2：3 progeny from a cross of an indica variety and a japonica variety. Gas chroma- tography-mass spectrometry （GC-MS） analysis revealed significant differences between parental lines in fatty acid com- position of brown rice oil, and 29 associated QTLs in F2 and/or F2：3 populations were identified throughout the rice genome, except chromosomes 9 and 10. Eight QTLs were repeatedly identified in both populations across different envi- ronments. Five loci pleiotropically controlled different traits, contributing to complex interactions of oil with fatty acids and between fatty acids. Nine rice orthologs of Arabidopsis genes encoding key enzymes in lipid metabolism co-localized with 11 mapped QTLs. A strong QTL for oleic （18：1） and linoleic （18：2） acid were associated with a rice ortholog of a gene encoding acyI-CoA：diacylglycerol acyltransferase （DGAT）, and another for palmitic acid （16：0） mapped similarly to the acyl- ACP thioesterase （FatB） gene ortholog. Our approach rapidly and efficiently identified candidate genes for mapped QTLs controlling fatty acid composition and oil concentration, providing information for improving rice grain quality by marker assisted selection.
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