Unveiling degradation mechanism of PAHs by a Sphingobium strain from a microbial consortium
作者机构：State Key Laboratory of Microbial MetabolismJoint International Research Laboratory of Metabolic and Developmental Sciencesand School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina CAS Key Laboratory of Quantitative Engineering BiologyGuangdong Provincial Key Laboratory of Synthetic Genomics and Shenzhen Key Laboratory of Synthetic GenomicsShenzhen Institute of Synthetic BiologyShenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina
出 版 物：《mLife》 (微生物（英文）)
年 卷 期：2022年第1卷第3期
页 面：287-302页
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学]
基 金：This study was supported by grants from the National Key R&D Program of China(2021YFA0909500) Shanghai Excellent Academic Leaders Program(20XD1421900) grants from the National Natural Science Foundation of China(32100075 and 32030004) “Shuguang Program”(17SG09)supported by the Shanghai Education Development Foundation and the Shanghai Municipal Education Commission
主 题：metagenomic binning analysis P450 polycyclic aromatic hydrocarbons ring‐hydroxylating dioxygenase Sphingobium
摘 要：Polycyclic aromatic hydrocarbons(PAHs)are a class of persistent pollutants with adverse biological effects and pose a serious threat to ecological environments and human *** previously isolated phenanthrene‐degrading bacterial consortium(PDMC)consists of the genera Sphingobium and Pseudomonas and can degrade a wide range of *** identify the degradation mechanism of PAHs in the consortium PDMC,metagenomic binning was conducted and a Sphingomonadales assembly genome with 100%completeness was ***,Sphingobium ***‐2,an efficient degrader of PAHs,was successfully isolated from the consortium *** SHPJ‐2 has powerful degrading abilities and various degradation pathways of high‐molecular‐weight PAHs,including fluoranthene,pyrene,benzo[a]anthracene,and *** ring‐hydroxylating dioxygenases,five cytochrome P450s,and a pair of electron transfer chains associated with PAH degradation in strain SHPJ‐2,which share 83.0%–99.0%similarity with their corresponding homologous proteins,were identified by a combination of Sphingomonadales assembly genome annotation,reverse‐transcription quantitative polymerase chain reaction and heterologous ***,when coexpressed in Escherichia coli BL21(DE3)with the appropriate electron transfer chain,PhnA1B1 could effectively degrade chrysene and benzo[a]anthracene,while PhnA2B2 degrade ***,these results provide a comprehensive assessment of strain SHPJ‐2 and contribute to a better understanding of the molecular mechanism responsible for the PAH degradation.
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