Fusarivirus accessory helicases present an evolutionary link for viruses infecting plants and fungi

作     者：Assane Hamidou Abdoulaye Jichun Jia Aqleem Abbas Du Hai Jiasen Cheng Yanping Fu Yang Lin Daohong Jiang Jiatao Xie Assane Hamidou Abdoulaye;Jichun Jia;Aqleem Abbas;Du Hai;Jiasen Cheng;Yanping Fu;Yang Lin;Daohong Jiang;Jiatao Xie

作者机构：State Key Laboratory of Agricultural MicrobiologyHuazhong Agricultural UniversityWuhan430070China Hubei Key Laboratory of Plant PathologyCollege of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan430070China Hubei Hongshan LaboratoryWuhan430070China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2022年第37卷第3期

页      面：427-436页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 

基　　金：financially supported by the Fundamental Research Funds for the Central Universities(2662018PY041) the Natural Science Foundation of China(31772111) the National Key Research and Development Program of China(2017YFD0201100)。 

主　　题：Fusarivirus Potyvirus Hypovirus Helicase Horizontal gene transfer(HGT) 

摘      要：A significant number of mycoviruses have been identified that are related to plant viruses,but their evolutionary relationships are largely unexplored.A fusarivirus,Rhizoctonia solani fusarivirus 4(RsFV4),was identified in phytopathogenic fungus Rhizoctonia solani(R.solani)strain XY74 co-infected by an alphaendornavirus.RsFV4 had a genome of 10,833 nt(excluding the poly-A tail),and consisted of four non-overlapping open reading frames(ORFs).ORF1 encodes an 825 aa protein containing a conserved helicase domain(Hel1).ORF3 encodes 1550 aa protein with two conserved domains,namely an RNA-dependent RNA polymerase(RdRp)and another helicase(Hel2).The ORF2 and ORF4 likely encode two hypothetical proteins(520 and 542 aa)with unknown functions.The phylogenetic analysis based on Hel2 and RdRp suggest that RsFV4 was positioned within the fusarivirus group,but formed an independent branch with three previously reported fusariviruses of R.solani.Notably,the Hel1 and its relatives were phylogenetically closer to helicases of potyviruses and hypoviruses than fusariviruses,suggesting fusarivirus Hel1 formed an evolutionary link between these three virus groups.This finding provides evidence of the occurrence of a horizontal gene transfer or recombination event between mycoviruses and plant viruses or between mycoviruses.Our findings are likely to enhance the understanding of virus evolution and diversity.