A tunable genome editing system of the prime editor mediated by dihydrofolate reductase

作     者：Shu Liu Xiaoyue Duan Feng Peng Yafang Wang Yang Liu Xiaoling Wan Jingfa Zhang Xiaosa Li Xiaodong Sun Shu Liu;Xiaoyue Duan;Feng Peng;Yafang Wang;Yang Liu;Xiaoling Wan;Jingfa Zhang;Xiaosa Li;Xiaodong Sun

作者机构：Department of OphthalmologyShanghai General Hospital(Shanghai First People’s Hospital)Shanghai Jiao Tong University School of MedicineShanghai 200080China Department of OphthalmologyShanghai Changhai HospitalShanghai 200043China Shanghai Key Laboratory of Ocular Fundus Diseases100 Haining RoadShanghai 200080China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报（英文版）)

年 卷 期：2023年第50卷第3期

页      面：204-207页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：surported by Research Foundation of Shanghai General Hospital 

主　　题：CRISPR/Cas genome system 

摘      要：The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein(CRISPR/Cas)system was discovered in bacteria and archaea as an adaptive immunity system to protect against exogenous DNA(Barrangou et al.,2007).This system has been developed into a powerful gene editing tool for eukaryotic genome manipulation that has therapeutic opportunities against hereditary diseases(Cong et al.,2013),especially monogenic diseases.