Functional characterization of key polyketide synthases by integrated metabolome and transcriptome analysis on curcuminoid biosynthesis in Curcuma wenyujin
作者机构：Key Laboratory of Elemene Class Anti-cancer Chinese Medicine of Zhejiang ProvinceEngineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang ProvinceSchool of PharmacyHangzhou Normal UniversityHangzhouZhejiang311121China School of Public HealthHangzhou Normal UniversityHangzhouZhejiang311121China
出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)
年 卷 期：2022年第7卷第3期
页 面：849-861页
核心收录：
学科分类：07[理学] 070303[理学-有机化学] 0703[理学-化学] 0836[工学-生物工程]
基 金：supported by the National Natural Science Foundation of China(82173919,82104320) the Natural Science Foundation of Zhejiang Province(LY21C050004,LQ22H280013).
主 题：Metabolome Curcuminoid Transcriptome Hydrogenated curcumin Polyketide synthase
摘 要：Leaf and tuber extracts of Curcuma wenyujin contain a mixture of curcuminoids.However,the curcuminoid constituents and their molecular mechanisms are poorly understood,and the relevant curcumin synthases remain unclear.In this study,we comprehensively compared the metabolite profiles of the leaf and tuber tissues of C.wenyujin.A total of 11 curcuminoid metabolites were identified and exhibited differentially changed contents in the leaf and tuber tissues.An integrated analysis of metabolomic and transcriptomic data revealed the proposed biosynthesis pathway of curcuminoid.Two candidate type III polyketide synthases(PKSs)were identified in the metabolically engineering yeasts,indicating that CwPKS1 and CwPKS2 maintained substrate and product specificities.Especially,CwPKS1 is the first type III PKS identified to synthesize hydrogenated derivatives of curcuminoid,dihydrocurcumin and tetrehydrocurcumin.Interestingly,the substitution of the glycine at position 219 with aspartic acid(G219D mutant)resulted in the complete inactivation of CwPKS1.Our results provide the first comparative metabolome analysis of C.wenyujin and functionally identified type III PKSs,giving valuable information for curcuminoids biosynthesis.
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