Advanced approach for antifungal susceptibility and characterization of resistance properties in clinical and environmental isolates of Cryptococcus species complex

作     者：Shayanki Lahiri Nagarathna Chandrashekar Shayanki Lahiri;Nagarathna Chandrashekar

作者机构：Department of NeuromicrobiologyNational Institute of Mental Health and Neuro SciencesBangaloreKarnatakaIndia Department of Computational Medicine and BioinformaticsUniversity of MichiganAnn ArborUSA 

出 版 物：《Infectious Medicine》 (感染医学（英文）)

年 卷 期：2022年第1卷第3期

页      面：147-153页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：funding to carry out the research(Grant Number:2121130717 Ref no:18-12/2011(ii)EU-V) 

主　　题：Cryptococcus neoformans/gattii Environmental isolates Clinical isolates Antifungal susceptibility CnAFR1 Amphotericin B resistance 

摘      要：Background:Meningitis due to Cryptococcus neoformans/gattii is a fatal infection affecting immunocompromised population *** B(AmB),fluconazole(FLC)and 5-flucytosine are the drugs of choice to treat the *** studied antifungal susceptibility pattern of clinical and environmental cryptococcal species using newer approach and analyze their resistant ***:Eighty clinical(54 *** and 26 ***)and 18 environmental(14 *** and 4 ***)isolates were subjected to antifungal susceptibility testing by automated(VITEK2C)*** inhibitory concentrations(MIC)were analyzed *** DNA of FLC resistant isolates was extracted and amplified to detect presence of CnAFR1 ***:*** showed 1.85%and 21.4%AmB resistance,and 1.85%and 28.5%FLC-resistance,whereas *** showed 25%and 50%FLC-resistance among clinical and environmental isolates *** values were significantly(p0.05)different for the isolates from 2 ***1 gene sequence analysis revealed phylogenetic relationship among the resistant ***:This pioneering study provides an insight into the sensitivity patterns of clinical and environmental cryptococcal isolates from south *** recent emergence of AmB-resistance may transpire as a challenge for the *** the clinical and environmental isolates are phylogenetically evolved from CnAFR1 gene of Filobasidiella neoformans,the resistance is most probably an inherent *** study emphasizes the need for speciation and antifungal susceptibility testing of cryptococcal isolates from clinical sources to institute appropriate antifungal therapy and to reduce the mortality and morbidity.