Current state and future perspectives of cytochrome P450 enzymes for C-H and C=C oxygenation

作     者：Yu Yan Jing Wu Guipeng Hu Cong Gao Liang Guo Xiulai Chen Liming Liu Wei Song 

作者机构：State Key Laboratory of Food Science and TechnologyJiangnan UniversityWuxi214122China School of Life Sciences and Health EngineeringJiangnan UniversityWuxi214122China 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2022年第7卷第3期

页      面：887-899页

核心收录：

学科分类：07[理学] 070304[理学-物理化学(含∶化学物理)] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：supported by the National Key R&D Program of China(Grant No.2021YFC2100100 and 2021YFC2102000) the General Program of National Natural Science Foundation of China(Grant No.22178146). 

主　　题：Biocatalysis P450 enzymes C-H and C=C oxygenation 

摘      要：Cytochrome P450 enzymes(CYPs)catalyze a series of C-H and C=C oxygenation reactions,including hydroxylation,epoxidation,and ketonization.They are attractive biocatalysts because of their ability to selectively introduce oxygen into inert molecules under mild conditions.This review provides a comprehensive overview of the C-H and C=C oxygenation reactions catalyzed by CYPs and the various strategies for achieving higher selectivity and enzymatic activity.Furthermore,we discuss the application of C-H and C=C oxygenation catalyzed by CYPs to obtain the desired chemicals or pharmaceutical intermediates in practical production.The rapid development of protein engineering for CYPs provides excellent biocatalysts for selective C-H and C=C oxygenation reactions,thereby promoting the development of environmentally friendly and sustainable production processes.