Genomics and proteomics of Apis mellifera filamentous virus isolated from honeybees in China

作     者：Dahe Yang Jun Wang Xi Wang Fei Deng Qingyun Diao Manli Wang Zhihong Hu Chunsheng Hou Dahe Yang;Jun Wang;Xi Wang;Fei Deng;Qingyun Diao;Manli Wang;Zhihong Hu;Chunsheng Hou

作者机构：Institute of Apicultural ResearchChinese Academy of Agricultural SciencesBeijing100093China State Key Laboratory of Virology and National Virus Resource CenterWuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China Institute of Bast Fiber CropsChinese Academy of Agricultural SciencesChangsha410205China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2022年第37卷第4期

页      面：483-490页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0906[农学-兽医学] 

基　　金：the Open Research Fund Program of the State Key Laboratory of Virology of China(Grant No.2019IOV004) the key Research Program of Frontier Sciences of Chinese Academy of Sciences(Grant No.QYZDJ-SSW-SMC021) the National Natural Science Foundation of China(Grant No.31900154 and 31572471)。 

主　　题：Apis mellifera filamentous Virus(AmFV) per os infectivity factor 4(PIF4) Genome sequence Proteomics Structural proteins Naldaviricetes 

摘      要：Apis mellifera filamentous virus(Am FV)is a large DNA virus that is endemic in honeybee colonies.The genome sequence of the Am FV Swiss isolate(Am FV CH–C05)has been reported,but so far very few molecular studies have been conducted on this virus.In this study,we isolated and purified Am FV(Am FV CN)from Chinese honeybee(Apis mellifera)colonies and elucidated its genomics and proteomics.Electron microscopy showed ovoid purified virions with dimensions of 300–500×210–285 nm,wrapping a 3165×40 nm filamentous nucleocapsid in three figure-eight loops.Unlike Am FV CH–C05,which was reported to have a circular genome,our data suggest that Am FV CN has a linear genome of approximately 493 kb.A total of 197 ORFs were identified,among which36 putative genes including 18 baculoviral homologs were annotated.The overall nucleotide similarity between the CN and CH–C05 isolates was 96.9%.Several ORFs were newly annotated in Am FV CN,including homologs of per os infectivity factor 4(PIF4)and a putative integrase.Phylogenomic analysis placed Am FVs on a separate branch within the newly proposed virus class Naldaviricetes.Proteomic analysis revealed 47 Am FV virionassociated proteins,of which 14 had over 50%sequence coverage,suggesting that they are likely to be main structural proteins.In addition,all six of the annotated PIFs(PIF-0–5)were identified by proteomics,suggesting that they may function as entry factors in Am FV infection.This study provides fundamental information regarding the molecular biology of Am FV.