Molecular and Serological Epidemiology of Foot-and-Mouth Disease Virus in North Region of Cameroon
Molecular and Serological Epidemiology of Foot-and-Mouth Disease Virus in North Region of Cameroon作者机构：Laboratoire National Vétérinaire LANAVET Garoua Cameroon Department of Animal Production Technology College of Technology The University of Bamenda Bamenda Cameroon Institute of Research in Tropical Ecology (IRET) Libreville Gabon Ecole des Sciences et de Médecine Vétérinaire Université de Ngaoundéré Ngaoundere Cameroon Foreign Animal Disease Research Unit United States Department of Agriculture Agricultural Research Service Plum Island Animal Disease Centre Greenport USA Agence Nationale de Sécurité Sanitaire de l’Alimentation de l’Environnement et du Travail Pierre et Marie Curie Maisons-Alfort France Department of Veterinary Preventive Medicine The Ohio State University Columbus USA Department of Veterinary Microbiology and Parasitology Faculty of Veterinary Medicine University of Maiduguri Maiduguri Nigeria
出 版 物：《Advances in Microbiology》 (微生物学（英文）)
年 卷 期：2022年第12卷第10期
页 面：579-595页
主 题：Foot-and-Mouth Disease Seroprevalence Non-Structural Protein Structural Protein ELISA Sedentary Cattle North Cameroon
摘 要：The serological prevalence of foot and mouth disease virus (FMDV) among the cattle population in the North region of Cameroon was determined using ELISA (Enzyme Linked Immunosorbent Assays) serological tests for structural as well as non-structural proteins. In these cattle, FMDV RNA was identified, amplified, sequenced and the sequences were used to construct a phylogenetic tree. A sedentary cattle population randomly selected from six veterinary centres in the North region was sampled twice, six months apart. High prevalence of FMDV antibody was recorded in the first (402/466 (85.84%)) and second (358/411 (86.90%)) sampling periods. There was no significant difference (P 0.05) in prevalence of FMDV antibody between the two sampling periods. Goudali and Peulh breeds of cattle and animals of three to five years old were the most infected with FMDV and mostly in the months of May and August. A seroprevalence of 100% (n = 14) of FMDV against serotypes A and O was observed in sera from convalescent animals in the study area. FMDV antigen detection ELISA showed a prevalence of 18/37 (48.65%) for serotypes SAT1 (8.1%), SAT2 (35.1%), A (10.8%) and O (2.7%) among the clinically infected animals. There was no significant difference (P 0.05) in prevalence of FMDV RNA between the sampling periods. A prevalence of FMDV RNA (17.5% (n = 120) and 16.7% (n = 240)) was observed among the sedentary animals that were sampled four to five months apart. FMDV RNA prevalence of 28/37 (75.6%) among clinically infected animals was also observed, thus confirming all the 12 outbreaks investigated. Sequence analysis of VP1 coding gene of the SAT2 serotype showed that it was homologous to the Libyan isolates (that caused epidemics in northern Africa in 2012) and also clustered with the serotypes isolated from both Nigeria and Sudan in 2007.
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