Porphyra haitanensis polysaccharide(PH)attenuates cell hyperplasia via remodeling the cross-talk between Hippo/YAP and mTOR pathways

作     者：Chong Wang Wanglei Lin Zhihua Sun Yiming Sun Yanbo Wang Linglin Fu Chong Wang;Wanglei Lin;Zhihua Sun;Yiming Sun;Yanbo Wang;Linglin Fu

作者机构：Food Safety Key Laboratory of Zhejiang ProvinceSchool of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China The Affiliated High School to Hangzhou Normal UniversityHangzhou 310030China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2023年第12卷第2期

页      面：424-430页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Key R&D Program of China(2017YFD0400203) the National Natural Science Foundation of China(31801452) the Zhejiang Provincial Science and Technology Foundation of China(2018C02025 and 2019C02069) the Zhejiang Provincial Natural Science Foundation of China(LGN21C200013) the China Postdoctoral Science Foundation(2019M662108) the Scientific Research Fund of Zhejiang Provincial Education Department(Y201942404)。 

主　　题：Porphyra haitanensis Polysaccharide Hippo mTOR Cancer 

摘      要：Porphyra polysaccharide is a special kind of nutrient showing multiple physiological functions including regulating cell proliferation,but the detailed mechanisms are not fully revealed,impairing its further development and applications.This work was to purify and characterize the Porphyra haitanensis polysaccharide(PH),investigate its physiological function,and demonstrate the underlying mechanisms.The purified PH was first characterized by Fourier-transform infrared spectroscopy.Then an intestinal epithelial cell model was established,in which PH significantly suppressed cell hyperplasia.Specifically,PH activated the Hippo/YAP pathway,which subsequently activated m TOR pathway,however m TOR activated Hippo in the absence of PH.Moreover,both the inhibition of Hippo by YAP1 knock-down and the suppression of m TOR by rapamycin impaired PH function.These results indicated that PH attenuated hyperplasia activity by remodeling the cross-talk between Hippo/YAP and m TOR pathways,which revealed potential targets and approaches for treating hyperplasia-related diseases and provided novel ways to utilize P.haitanensis as well as other related functional foods.