Structural characterization and immunostimulatory activity of a water-soluble polysaccharide from abalone(Haliotis discus hannai Ino)muscle

作     者：Linfan Shi Gengxin Hao Jun Chen Junling Wang Wuyin Weng Linfan Shi;Gengxin Hao;Jun Chen;Junling Wang;Wuyin Weng

作者机构：College of Ocean Food and Biological EngineeringJimei UniversityXiamen 361021China Engineering Research Center of the Modern Technology for Eel IndustryMinistry of EducationXiamen 361021China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2023年第12卷第2期

页      面：495-502页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 083204[工学-水产品加工及贮藏工程] 

基　　金：financial support received from the National Key R&D Program of China(2021YFD2100200/2021Y FD2100202) National Natural Science Fund(31571835),Fujian Key Project of Natural Science Foundation(2019J02013) the Opening Project of Fujian Provincial Engineering Technology Research Center of Marine Functional Food(Z820239) 

主　　题：Polysaccharide Abalone muscle Structural characterization Immunostimulatory activity Nuclear magnetic resonance spectra 

摘      要：A water-soluble polysaccharide from abalone muscle(AMPP)was *** contents of carbohydrate,protein,uronic acid,and sulfate in AMPP were 83.5%,0.5%,2.7%,and 2.6%,***-performance liquid chromatography analysis indicated that AMPP was homogeneous and had an average molecular weight of approximately 3.2 *** main monosaccharides of AMPP were glucose(Glc)and mannose with a molar ratio of 99.7:*** structural characteristics of AMPP were elucidated through methylation analysis,Fourier transform infrared spectroscopy,and nuclear magnetic resonance *** linkages of AMPP consisted of terminal,1,4-linked,1,6-linked,and 1,4,6-linked Glcp with a molar ratio of 3.1:7.2:1.0:*** one repeat unit of the proposed AMPP structure,the backbone chain was composed of eight 1→4 glycosidic bonds and one 1→6 glycosidic bond,with three branch chains linked by 1→6 glycosidic *** addition,AMPP was found to possess potent immunostimulatory activity via rising phagocytosis of RAW264.7 cells and promoting secretion of TNF-α.